High-Performance Liquid Chromatography Using Monolithic Column for Simultaneous Determination of Oxymetazoline Hydrochloride and Xylometazoline Hydrochloride in Nasal Spray Formulations
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Abstract
In this research, a simple and rapid high-performance liquid chromatography for simultaneous determination of oxymetazoline hydrochloride and xylometazoline hydrochloride in nasal spray formulations was developed. The separation was conducted in monolithic column (C18, 100 mm x 4.6 mm). A mixture of acetonitrile (28% by volume) and 0.01% phosphoric acid (72% by volume) was used as the optimal mobile phase, with the flow rate of 2.50 mL/min. All analytes were detected at 220 nm. Under the optimized conditions, oxymetazoline hydrochloride and xylometazoline hydrochloride could be completely separated within 2 min. Calibration curves for standard solution of oxymetazoline hydrochloride and xylometazoline hydrochloride were in the range of 0.50-10.00 mg/L with R2 > 0.9987. Limits of detection of oxymetazoline hydrochloride and xylometazoline hydrochloride were 0.03 and 0.04 mg/L, respectively. The proposed method can be used for routine analysis of oxymetazoline hydrochloride and xylometazoline hydrochloride in nasal spray formulations.
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