Development of UV Chamber for Antibacterial Activity by the Faculty of Engineering, Ubon Ratchathani University
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Abstract
This study aims to examine the ability of UV chamber developed by the Faculty of Engineering, Ubon Ratchathani University to inhibit Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus) and Extended Spectrum Beta Lactamase Escherichia coli (ESBL E. coli) that were used as representatives of gram negative bacteria, gram positive bacteria, spore producing bacteria and drug resistant bacteria, respectively. When the tested bacteria with the initial concentration of 0.5 McFarland were streaked on BHI agar and exposed to UV for 10 min, all tested bacteria were inhibited. However, when the same experiment was performed using each bacteria with the initial concentration of 0.5 McFarland but decreasing the UV exposure time to 2, 3, 4 and 5 min, it was found that exposure to UV for 3, 4 and 5 min could inhibit all of the tested bacteria while exposure to UV for 2 min could inhibit only S. aureus and ESBL E. coli but not B. cereus and E. coli. Moreover, it was also found that exposure to UV for 3 min could inhibit all of the tested bacteria with the initial concentrations of 1, 2, 3 and 4 McFarland. From this study, it is suggested that the most effective exposure time for the application of the UV chamber to inhibit all types of bacteria would be at least 3 min.
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