Simultaneous Analysis of Tramadol Hydrochloride and Paracetamol in Tablets by Capillary Electrophoresis
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Abstract
In this paper, capillary electrophoresis method for simultaneous analysis of tramadol hydrochloride and paracetamol in tables was developed. Optimal conditions for separation were capillary length of 32 cm, borate buffer of 10 mM (pH 9.5), and injection at 50 mbar for 3 sec. Detection wavelength was set 215 nm. Under the optimal conditions, analysis was carried out in less than 2 min. Migration times of tramadol hydrochloride and paracetamol were 1.161 and 1.615 min, respectively. Calibration curve were linear over the concentration range 5.0-100.0 mg/L with R2 greater than 0.9996. Limits of detection for tramadol hydrochloride and paracetamol were 1.05 and 0.60 mg/L, respectively. Limits of quantitation for tramadol hydrochloride and paracetamol were 3.50 and 2.00 mg/L, respectively. Percent recoveries were in range 96.2-101.3. The development method could be applied to the simultaneous analysis of tramadol hydrochloride and paracetamol in tablets.
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