The two-phase microchannel flow study of chicken blood on lab-on-a-chip

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Published: Oct 11, 2018
Keywords:
LOC Lab-on-a-Chip Chicken Blood Two-Phase Flow PDMS DEP Force

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A. Pramuanjaroenkij
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
K. Thanomsit
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
B. Chaiburi
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
G. Phonong
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
A. Khanaphol
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
W. Phutthasawong
Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000 Thailand
T. Maturos
Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, Pathumthani 12120 Thailand
T. Lomas
Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, Pathumthani 12120 Thailand
A. Tuantranont
Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, Pathumthani 12120 Thailand
S. Kakaç
Department of Mechanical Engineering, TOBB University of Economics and Technology, Ankara, 06560 Turkey
C. Thipyopas
Department of Aerospace Engineering, Kasetsart University, Bangkok 10900 Thailand

Abstract

In previous study, we introduce problems in assembly the polydimethylsiloxane (PDMS) lab-on-a-chip (LOC) devices from literatures; a manual assembly by using acrylic plates and an adhered assembly by using a plasma cleaner. Since electrodes in the devices had been designed to provide a strong dielectrophoretic force by using sputtering and electroplating techniques, the thick electrodes were used. The PDMS LOC was also aimed to use with biological samples to study the different flow patterns of the samples prepared from chicken blood and to solve a PDMS hydrophobic problem from our previous study where the sample cannot flow through a channel of the adhered assembly LOC. Since the chicken blood contains of whole blood and plasma, the sample can be considered as a combination of particle and fluid parts or the two-phase flow. The channels on the PDMS LOC having dimensions of the order of microns can be considered as microchannels; Knudsen number is used to distinguish flow regimes. From the experiment study, the flow problem has still existed in the adhered method but the flow problem did not exist on the manual method with a new-acrylic-plate set. Slip flow occurred at the leak areas because interactions between the sample and walls became significant. Different flow patterns occurred in the channels of workable LOCs in different channel distances. No slip (continuum) flow occurred at the middle path where pressure drop was decreased. The hydrophobic problem was solved by using Bovine Serum Abumin solution to reduce PDMS hydrophobic behavior and this solution did not affect red blood cells because of none damaged cells.

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How to Cite
Pramuanjaroenkij, A., Thanomsit, K., Chaiburi, B., Phonong, G., Khanaphol, A., Phutthasawong, W., Maturos, T., Lomas, T., Tuantranont, A., Kakaç, S., & Thipyopas, C. (2018). The two-phase microchannel flow study of chicken blood on lab-on-a-chip. Journal of Research and Applications in Mechanical Engineering, 1(2), 43–47. Retrieved from https://ph01.tci-thaijo.org/index.php/jrame/article/view/150196
Issue
Vol. 1 No. 2 (2012)
Section
RESEARCH ARTICLES

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