CONCENTRATION OF -AMYRIN AND ANTIBACTERIAL ACTIVITY AGAINST PROPIONIBACTERIUM ACNES OF ETHANOLIC EXTRACTS FROM SIAM WEED (CHROMOLAENA ODORATA L.) LEAVES
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Abstract
The objectives of this study were to evaluate the concentration of -amyrin in an ethanol extracts of Siam weed (Chromolaena odorata L.) leaves and to test the bioactivity of an ethanol extracts from the Siam weed leaves with inhibit P. acnes (DMST14916). The results of the study showed that concentration of
-amyrin in ethanol extracts of the Siam weed leaves was 163.1 mg/g of dry leave, and retention time was 4.355 minute (peak of standard
-amyrin at 4.251 minute). In the part of the bioactivity test on P. acnes showed that minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Siam weed leaves extracts were 0.78±0.062 mg/ml and 1.56±0.017 mg/ml, respectively. MIC of Siam weed leaves extracts was higher than that of Tetracycline HCL, mangosteen pericarp extracts and liquorice stem bark extracts, but lower than that of cassava leaves extracts. MBC of Siam weed leaves extracts was higher than that of Tetracycline HCL and mangosteen pericarp extracts, but lower than that of liquorice stem bark extracts and cassava leaves extracts. In addition, disc diffusion method (clear zone test) at 0.2 mg/ml of Siam weed leaves extracts concentration revealed that radius of clear zone of the Siam weed extracts on P. acnes was 14.67±0.471 mm (diameter of paper disc is 6 mm). The results of the study demonstrated that it can be used as a good anti- P. acnes agent.
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อดิเทพชัยย์การณ์ ภาชนะวรรณ และ ปราณีต งามเสน่ห์. (2553). ประสิทธิภาพของสารสกัดจากเปลือกมังคุดในการยับยั้งเชื้อ Streptococcus agalactiae ที่เป็นแบคทีเรียก่อโรคในปลา. วารสารวิจัยเทคโนโลยีการประมง, 4(1), 103-110.
Abass, A. M. (2018). Preparation and application of tetracycline hydrochloride liquid membrane electrodes. Journal of Al-Nahrain University (Science), 21(2), 73-80.
Adiguzel, A., Ozer, H., Killic, H., & Cetin, B. (2007). Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. Czech Journal of Food Sciences, 25(2), 81-89.
Aisha, A. F. A., Abu-Salah, K. M., Ismail, Z., & Majid, A. M. S. A. (2012). In vitro and in vivo anti-colon cancer effects of Garcinia mangostana xanthones extract. BMC Complementary and Alternative Medicine, 12(104), 1-10.
Basri, D. F., & Fan, S. H. (2005). The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian Journal of Pharmacology, 37(1), 26-29.
Bouda, H., Tapondjou, L. A., Fontem, D. A., & Gumedzoe, M. Y. D. (2001). Effect of essential oils from leaves of Ageratum conyzoides, Lantana camara and Chromolaena odorata on the mortality of Sitophilus zeamais (Coleoptera, Curculionidae). Journal of Stored Products Research, 37(2), 103-109.
Brandas, S. S., Vik, A., Friedman, L., & Koiter, R. (2005). Biofilm: the matrix revisited. Trends Microbiology, 13(1), 20-26.
Brown, G. D. (1998). The biosynthesis of steroids and triterpenoids. Natural Product Reports journal, 15, 653-695.
Garcia-Risco, M. R., Mouhid, L., Salas-Perez, L., Lopez-Padilla, A., Santoyo, S., Jaime, L., Molina, A. R., Reglero, G., & Fornari, T. (2017). Biological activities of Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) plant extracts. Plant Foods for Human Nutrition, 72(1), 96-102.
Harizon, Pujiastutib, B., Kurniab, D., Sumiarsab, D., Shionoc Y., & Supratman, U. (2015). Antibacterial triterpenoids from the bark of Sonneratia alba (Lythraceae). Natural Product Communications, 10(2), 277-280.
Hassan, I. A. S., Hassan, M. A., Embarek, M. S., Attallah, D. A., Mokhtar, M. A. E., & Eldin, G. M. A. (2015). Antibiotic susceptibility patterns of Propionibacterium acnes isolated from Acne Vulgaris in Assiut University hospitals, Egypt. Egyptian Journal of Medical Microbiology, 24(4), 67-72.
Hudzicki, J. (2009). Kirby-Bauer disk diffusion susceptibility test protocol. American Society for Microbiology, Washington, D.C.
Julianti, E., Rajah, K. K., & Fidrianny, I. (2017). Antibacterial activity of ethanolic extracts of Cinnamon bark, honey, and their combination effects against acne causing bacteria. Scientia Phamaceutica, 85(2), 1-8.
Khumsupan, P., & Gritsanapan, W. (2014). Anti-acne activity of Garcinia mangostana L.: a review. Plant Science Today, 1(3), 147-150.
Kiplimo, J. J. (2016). A review on the biological activity and the triterpenoids from the genus Vernonia (Asteraceae Family). International Research Journal of Pure and Applied Chemistry, 11(3), 1-14.
Luangnarumitchai, S., Lamlertthon, S., & Tiyaboonchai, W. (2007). Antimicrobial activity of essential oils against five strains of Propionibacterium acnes. Mahidol University Journal of Pharmaceutical Sciences, 34(1-4), 60-64.
Miklasinska-Majdanik, M., Kepa, M., Wojtyczka, R. D., Idzik, D., & Wasik, T. J. (2018). Phenolic compounds diminish antibiotic resistance of Staphylococcus aureus clinical strains. International Journal of Environmental Research and Public Health, 15(10), 1-18.
Mustarichie, R., Sulistyaningsih, S., & Runadi, D. (2020). Antibacterial activity test of extracts and fractions of cassava leaves (Manihot esculenta Crantz) against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes causing acne. Hindawi International Journal of Microbiology, DOI:10.1155/2020/1975904.
Olajide, O. A., Awe, S. O., Makinde, J. M., Ekhelar, A. I., Olusola, A., Morebise, O., & Okpako, D. T. (2000). Studies on the anti-inflammatory, antipyretic and analgesic properties of Alstonia boonei stem bark. The Journal of Ethnopharmacology, 71(1-2), 179-186.
Oliveira, F. A., Chaves, M. H., Almeida, F. R., Lima, R. C., Jr, Silva, R. M., Maia, J. L., Brito, G. A., Santos, F. A., & Rao, V. S. (2005). Protective effect of alpha- and beta-amyrin, a triterpene mixture from Protium heptaphyllum (Aubl.) March. trunk wood resin, against acetaminophen-induced liver in-jury in mice. Journal of Ethnopharmacology, 98, 103-108.
Parsaeimehr, A., Martinez-Chapa, S. O., & Parra-Saldivar, R. (2017). Medicinal plants versus skin disorders: a survey from ancient to modern herbalism clinical microbiology. Diagnosis, Treatments and Prophylaxis of infections, 2, 205-221.
Pothitirat, W., Chomnawang, M. T., & Gritsanapan, W. (2009). Anti-acne inducing bacteria activity and -mangostin content of Garcinia mangostana fruit rind extracts from different provenience. Songklanakarin Journal of Science and Technology, 31(1), 41-47.
Rouw, A. D. (1991). The invasion of Chromolaena odorata (L.) King & Robinson (ex Eupatorium odoratum), and competition with the native flora, in the rain forest zone, south-west cote d’ Ivoire. Journal of Biogeography, 18, 13-23.
Sporn M. B., Liby, K. T., Yore, M. M., Fu L., Lopchuk, J. M., & Gribble, G. W. (2011). New synthetic triterpenoids: potent agents for prevention and treatment of tissue injury caused by inflammatory and oxidative stress. Journal of Natural Products, 74, 537-545.
Stefanovic, O., & Comic, L. (2011). Inhibitory effect of Cytisus nigricans L. and Cytisus capitatus Scop. on growth of bacteria. African Journal of Microbiology Research, 5(27), 4725-4730.
Triratana, T., Suwannuraks, R., & W. Naengchomnong. (1991). Effect of Eupatorium odoratum on blood coagulation. Journal of the Medical Association of Thailand, 74(5), 283-287.
Venkata, R. B., Samuel, L. A., Saradhi, M. P., Rao, B. N., Krishna, A. N. V., Sudhakar, M., & Radhakrishnan, T. M. (2012). Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian Journal of Pharmaceutical and Clinical Research, 5(2), 99-106.
Vitelli, J. S., Panetta, F. D., Madigan, B. A., & Van Haaren, P. E. (2018). Chemical options for the control of Siam Weed (Chromolaena odorata) in natural ecosystems. Ecological Management & Restoration, 19(1), 88-93.
Wagh, S. J., Gujar, J. G., & Gaikar, V. G. (2012). Experimental and modeling studies on extraction of amyrins from latex of mandar (Calotropis gigantea). Indian Journal of Chemical Technology, 19, 427-433.