ANTI-ANGIOGENESIS ACTIVITIES OF MULBERRY ROOT BARK EXTRACTS.
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Abstract
The aims of this study were to determine anti-angiogenesis activity of mulberry root bark extracts; hexane, dichloromethane and methanol. Betulinic acid and β-sitosterol were isolated from the hexane extract. All extracts and fractions obtained from column chromatography were tested for cytotoxicity against HUVECs cell. The concentrations which did not show any toxicity were in the range of 0.1-1.0 µg/ml, therefore these concentrations were used for determination of anti-angiogenesis activity. The sub-fractions of hexane extract gave better activity than the isolated pure compounds. The percentage of inhibition were 77.8-93.3% while betulinic acid and β-sitosterol gave only 73-85.8%. The dichloromethane extract without hexane solubles gave 69.4-86.7 percentage of inhibition. The dichloromethane solubles from methanolic extract (MeOH/CH2Cl2) gave the best antiangiogenesis activity, 95.4 %. Therefore, the antiangiogenesis activity of mulberry root bark extracts were due to betulinic acid and β-sitosterol in combination with other substances. The best activity was found with (MeOH/CH2Cl2) extract at 0.1 µg/ml in which betulinic acid and β-sitosterol were absent.
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