BIOLOGICAL ACTIVITY OF CARISSA CARANDAS LINN. YOUNG FRUIT EXTRACT ON HEPG2, HELA AND HUMAN KERATIONCYTE CELLS HACAT

Abstract

            Carunda fruit was local fruit which Thai people pay more attention lately because it has health benefits and can be used to treat diseases. In order to make more effective medical uses of carunda fruit, the biological activities of the rough extract of the unripe carunda fruit in aqueous layer on HepG2, HeLa and HaCaT cells were examined. The antioxidant research activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The results reveal that the IC₅₀ were at 88.05 ± 0.06 µg/mL and 0.25±0.28 mg/mL respectively. In addition, this research also tested the effects of carunda fruit at concentrations of 0, 0.01, 0.05, 0.10, 0.50 and 1.00 mg / mL with all 3 cell types for 24 and 48 hours. The cell survival rate was determined afterward by using the MTT assay method.  It was found that when comparing to the controls, HepG2 cells of 0.01-1.00 mg / mL at all intervals had a statistically significant decrease in cell survival (P <0.05). In testing with Hela cells, 0.05-1.00 mg /mL concentrations for 48 hours had a statistically significant decreased in cell survival (P <0.05). The HaCaT cells at the concentration of 0.01 mg/mL had lower survival rate than 50% compared to the controls (P <0.05). It was found that the rough extract of the unripe carunda fruit had different cytotoxic responses which may be useful in the development of important substances for use in the prevention of various diseases Including cancer in the future