EFFECT OF CULTURE MEDIA AND SUCROSE CONCENTRATIONS ON IN VITRO YOUNG SHOOTS STORAGE OF MUSA (AA GROUP) ‘KHAI KAMPHAENG PHET’
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Abstract
In vitro shoot tips of Musa (AA group) ‘Khai Kamphaeng Phet’ were cultured on MS, ½ MS and ¼ MS combined with various sucrose concentrations at 0, 10, 20 and 30 gram/litre for 24 weeks. The research found that the highest percentage of viability (100%) could be obtained on ½ MS and ¼ MS medium supplemented with 30 gram/lite of sucrose. The shoots were then subcultured onto MS medium without hormone for regeneration recovery testing for 12 weeks. The results revealed that the highest regeneration percentage (20%) could obtained in all MS, ½ MS and ¼ MS augmented with 30 gram/lite sucrose.
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