NUTRITION AND SOME PATHOGENIC BACTERIA CONTAMINATION OF COW’S INTESTINAL CONTENTS

Abstract

This research aimed to study nutrition and contamination of some pathogenic bacteria in cow’s intestinal contents. Randomly sampling from slaughterhouses, beef shop in municipality market and sideways in Amphoe Mueang, Sakon Nakhon were used as the sample. The study of nutritional value, found the highest moisture content of 87.02±0.10% followed by the carbohydrate (6.35±0.15%), fiber (2.27±0.08%), ash (1.81±0.22%), protein (1.75± 0.06%) and fat (0.80±0.13%), respectively. The determination of some pathogenic bacteria contamination in cow’s intestinal contents, showed that the highest level of contamination was in the rainy season with 1.1×10⁶ CFU/mL, followed by summer and winter, which are 3.5×10⁵ CFU/mL and 2.2×10⁵ CFU/mL, respectively. For this contamination, found Escherichia coli in all samples of cow’s intestinal contents with a higher amount than the MPN E. coli criteria defined. Determination of Staphylococcus aureus, exposed the contamination S. aureus in lower level than the standards defined (<100 MPN/mL) in sample that collected from four sources within rainy season. Whereas Salmonella sp. was not found in all samples. For the study of the dominant microbial species considered, found seven isolates that classified into four isolates of Gram negative bacilli (RA-1, WA-2, SA-3 and SB-1), two isolates of Gram positive bacilli (WA-1 and SA-1) and one isolate of Gram positive cocci (SA-2).