PROTEOMIC EXPRESSION OF PHYSIOLOGICAL DISORDER SYNDROME IN LONGAN (DIMOCARPUS LONGAN L. “DAW”) ON INITIAL STAGE USING ONE-DIMENSIONAL ELECTROPHORESIS COUPLED WITH LC-MS/MS
Keywords:Longan, Leaves, Peel, Seeds, Physiological Disorder Syndrome, Electrophoresis
Comparative study on leaves, peels and seeds of longan (Dimocarpus longan Lour. var. Daw) at Prao and Mae Taeng district in Chiang Mai, was compared between normal and physiological disorder syndrome in longan on initial fruit by non-native conditions in electrophoresis gel with staining gel by Coomassie brilliant blue R-250 following liquid chromatography mass spectrometry (LC-MS/MS). The results showed that plasma membrane-associated cation-binding protein 1 (19.5 kDa) was found in decreased protein on abnormal longan at Prao district at 20 weeks after fruition. The vegetative storage protein (25.2 kDa) was also found at the seed in Prao district similar to the results from Mae Taeng district presented biomarkers at leaves, peel and seed since disorder on initial stage (20 weeks after fruition) to harvest stage (26 weeks after fruition). It was noted that leaves and seeds of abnormal longan is higher in protein than normal longan but lower in protein than in peel. The abnormal longan in seed at 24 weeks after fruition was not found three biomarkers, triosphosphate isomerase-like protein type 1 (27.4 kDa), hypothetical protein (24.8 kDa) and peroxidase 4 (39.7 kDa). These proteins may be important protein in part of the recovery process of abnormal longan.
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