ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF MUSHROOMS FROM A COMMUNITY ENTERPRISE IN LAMPANG PROVINCE, THAILAND
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The objectives of this research were to evaluate the antibacterial and antioxidant activities of ethanolic and aqueous extracts of five mushrooms cultivated by a community enterprise in Lampang Province, Thailand. Five mushrooms including Lentinus edodes, Lentinus polychrous, Lentinus squarrosulus, Pleurotus ostreatus, and Dictyophora indusiata were identified through ITS sequencing analysis. The ethanolic and aqueous extracts of these mushrooms were analyzed using the agar disc diffusion method and the DPPH radical scavenging activity assay, followed by statistical analysis using a one-way analysis of variance (ANOVA). Results showed that the ethanolic extract of L. squarrosulus exhibited the antibacterial activity against Proteus mirabilis DMST8212, Enterobacter aerogenes DMST8841, and Salmonella typhimurium DMST562, with clear zone diameters of 12.83 mm, 6.83 mm, and 6.00 mm, respectively. The growth of Staphylococcus aureus DMST8840 was inhibited by the ethanolic extracts of P. ostreatus and L. edodes with clear zone diameters of 6.67 mm and 6.00 mm, respectively. The ethanolic extract of L. polychrous inhibited P. mirabilis growth, resulting in a clear zone diameter of 11.33 mm. Antioxidant activity varied among species. The highest DPPH radical-scavenging activity was observed in ethanolic L. edodes and aqueous L. polychrous extracts at 10 mg/ml, with %DPPH reduction values of 92.23% and 96.69% respectively. The IC50 values ranged from 4.951 to 7.952 mg/ml for ethanolic extracts, showing significant differences, and from 0.003 to 0.014 mg/ml for aqueous extracts, showing no significant differences. These findings highlight the potential of these mushrooms as natural antibacterial agents and antioxidants, offering opportunities for community enterprises to enhance income and sustainability through value-added products like dietary supplements, medicines, and cosmetics.
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