Cellulolytic Lactic Acid Probiotic Bacteria from Cricket Gut: Antioxidant and α-Amylase Inhibitory Activities of Their Exopolysaccharide
DOI:
https://doi.org/10.55674/cs.v18i1.262749Keywords:
Cricket gut, Lactic acid bacteria, Antioxidant activity, α-Amylase inhibitionAbstract
This study aimed to isolate cellulolytic lactic acid bacteria (LAB) with probiotic properties from the gut of crickets and to investigate the antioxidant and α-amylase inhibitory activities of the exopolysaccharide (EPS) produced by the selected bacterial strains. Cricket samples were collected from four farms in Ubon Ratchathani Province, Thailand. A total of 14 LAB isolates were obtained and characterized based on their morphological and biochemical properties. The isolates were subsequently screened for their cellulolytic activity, and only one isolate, designated FF02041, exhibited cellulase enzyme production. Comparative analysis of the 16S rRNA gene sequence (1,282 bp) identified FF02041 as Lactobacillus sp. This isolate was further evaluated for probiotic potential and demonstrated tolerance to both acidic conditions and bile salts. The EPS extracted from isolate FF02041 exhibited DPPH and ABTS free radical scavenging activities of 15.93% and 25.59%, respectively, at a concentration of 10 mg mL-1. Additionally, the EPS showed ferric reducing antioxidant power (FRAP) of 21.74 ± 0.06 mg Fe²⁺ equivalents per gram of EPS. Furthermore, at the same concentration (10 mg mL-1), the EPS displayed α-amylase inhibitory activity with an inhibition rate of 49.61%, corresponding to 22.74 ± 0.09 mg acarbose equivalents (AE) per gram of EPS. These findings highlight the cricket gut represents a novel reservoir of functional probiotics and bioactive EPS with strong potential for nutraceutical and functional food development.
GRAPHICAL ABSTRACT
HIGHLIGHTS
- Isolation of Cellulolytic Lactic Acid Probiotic Bacteria from Cricket Gut
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