PLANT GROWTH PROMOTING PROPERTIES OF BACTERIAL ISOLATES FROM SLUDGE OF BIOGAS PRODUCTION PROCESS
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Abstract
The objective of this research was to isolate bacteria from biogas sludge and investigate the plant growth promoting qualifications. The sample were 77 bacterium’s isolates, consisting of 56 isolates of gram-positive and 21 isolates of gram-negative. The qualifications testing of plant growth-promoting consisted of nitrogen fixation, siderophore production, phosphate solubilization, and indole-3-acetic acid production. The research results show that 52 isolates could be able to fix nitrogen on nitrogen-free solid malate medium (NFM) and were divided into 2 groups. The first group included 27 isolates which grown on NFM and changed the color of bromothymol blue. The second group contained 25 isolates which could be able to grow on NFM but not be able to change the color of bromothymol blue. On Chrom Azurol S (CAS) medium, bacteria were found in capable of forming a total of 70 isolates of siderophores. Phosphate solubility tests on GYA double-layered agar medium revealed that 24 isolates were able to grow and form clear blue regions around colonies, and the capability in producing Indole-3-acetic acid test found that there were 67 isolates were capable in producing Indole-3-acetic acid.
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