In vitro anti-pathogenic activity of composite materials made of activated carbon from bamboo containing silver nanoparticles
DOI:
https://doi.org/10.55674/cs.v17i1.258193Keywords:
Nanotechnology, Bamboo charcoal, Composite materials, Physical ReactivationAbstract
Pathogenic bacterial contamination has become a major concern for public health and the environment. However, the development of anti-pathogenic materials remains exceptionally challenging. This study examined the anti-pathogenic activities of composite materials by combining silver nanoparticles with activated carbon from bamboo charcoal. This was done to improve the overall qualities of the materials, avoid agglomeration, and maintain their original properties. The experiments sought suitable conditions for producing activated carbon from bamboo charcoal using physical reactivation under high-temperature steam. This greatly increased the specific surface area. Then, the material was combined with silver nanoparticles to test its anti-pathogenic activity against two common pathogenic bacteria, Escherichia coli DMST 12743 and Staphylococcus aureus DMST 19381. The findings revealed that the optimum time for producing activated carbon under high-temperature steam at 650 °C was 30 min. Anti-pathogenic activity was determined using an agar disc diffusion method. Testing included determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using broth microdilution and drop plate methods. These tests showed a greater anti-pathogenic effect against the Gram-negative bacterium. The zones of inhibition (ZOI) were 12.00±1.00 mm and 10.67±0.29 mm, respectively, against E. coli and S. aureus. MIC values of 1.56 µg/mL resulted with MBC values of 25 µg/mL. These composite materials have antimicrobial properties. This promotes nanotechnology and activated carbon use from agricultural waste for commercial products. These products can be employed in various treatment systems and medical devices for environmental safety and public health enhancement. Our approach demonstrates the development of nanotechnology and biomedical science integrated with green technology.
GRAPHICAL ABSTRACT
HIGHLIGHTS
- Silver nanoparticles from the green synthesis process were coated onto activated carbon derived from bamboo waste under optimized conditions.
- The resulting composite exhibits antibacterial properties and can be scaled for commercial applications.
- This eco-friendly approach promotes sustainable waste management and offers a cost-effective solution for antimicrobial products.
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