Evaluation of genetic variation in longan (Dimocarpus longan Lour) by high annealing temperature random amplified polymorphic DNA (HAT−RAPD)
DOI:
https://doi.org/10.55674/cs.v15i2.250017Keywords:
Dimocarpus longan Lour, High annealing temperature RAPD, Genetic variation, Molecular markerAbstract
Genetic relationships among 20 longan varieties (Dimocarpus longan Lour.) collected from Chiang Rai and Lampang province; Thailand, Vietnam and China were investigated using high annealing temperature random amplified polymorphic DNA (HAT−RAPD) technique. Ten primers, OPA 13, OPAK10, OPB 18, OPG 13, OPW 06, OPW 09, OPX 01, OPX 15, OPZ 01 and OPAK 10, produced a total of 305 RAPD markers. All primers generated a high degree of polymorphism, ranging in molecular weight from 100 to 2,500 bp. Hierarchical cluster analysis, based on unweighted neighbor−joining method classified the 20 varieties into 3 major groups. The first two groups could be divided into 2 subgroups and the third group contained only one subgroup. Our data revealed that members in the first group are Daw types.
The Narapirom and Petchsakorn varieties exhibited close relatedness, suggesting that they are originated from the same variety. In addition, this technique also detected variation among longan varieties due to selective breeding.
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