The analysis of nutritional value, total phenolic and flavonoid contents, and antioxidant activities from the ethanolic extracts of the roasted broken brown rice powder


  • Kulwadee Suwannatrai Program of Biology, Faculty of Science and Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon, 47000 Thailand
  • Krittiyanee Namwongsa Program of Chemistry, Faculty of Science and Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon, 47000 Thailand
  • Natenapa Phanomkhet Program of Biology, Faculty of Science and Technology, Sakon Nakhon Rajabhat University, Sakon Nakhon, 47000 Thailand
  • Hathaichanok Nuntapanich Program of Environmental Science, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani, 34000 Thailand
  • Wuttichai Roschat Program of Chemistry, Faculty of Science and Technology, Sakon Nakhon Rajabhat University



Roasted broken brown rice powder, Ethanolic extracts, Total phenolic and flavonoid contents, Antioxidant activities, Nutritional value


The objective of this study was to determine the nutritional value, total phenolic and flavonoid contents, and antioxidant activities of the roasted broken brown rice powder. The roasted broken brown rice powder was dried at 60 °C for 3 h in a hot air oven and extracted with absolute ethanol by using ultrasonic technique. Then, the sample was filtered to separate the residue and mixture solution and dried to remove the solvent by rotary evaporator for obtaining the ethanolic crude extract. Finally, the crude extract was collected and determined proximate composition, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity. The results revealed that the percentage crude extract of the extraction yield was 0.47% by weight with crude carbohydrate (74.47% w w−1), crude protein (8.11% w w−1), and fat (0.05% w w−1), respectively. The TPC and TFC were found at an average of 20.40 ± 0.37 mg GAE g−1 crude extract and 4.58 ± 0.19 mg QE g−1 crude extract, respectively. Moreover, the sample has highly efficient antioxidants activity similar to a standard Trolox solution. Therefore, it was reasonably concluded that the roasted broken brown rice powder product was rich in beneficial nutrients for the health effects and represented adding value to a by–product of rice production.


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B. Yadav, V. Jindal, Changes in head rice yield and whiteness during milling of rough rice (Oryza sativa L.), J. Food Eng. 86 (2008) 113 − 121.

A. Aadil, M. Shahzad, A Faiza, K. Ayesha, R. Shakeela, N. Sumera, Effect of processing on nutritional value of rice (Oryza sativa), World J. Medical Sci. 6(2) (2011) 68 − 73.

Y. Bao-Min, C. Peng, S. Guo Xin, Distribution of elements and their correlation in Bran, polished rice and whole grain, Food Sci. Nutr. 8 (2020) 982 – 992.

R. Zhang, S.A. Khan, J. Chi, Z. Wei, Y. Zhang, Y. Deng, L. Liu, M. Zhang, Different effects of extrusion on the phenolic profiles and antioxidant activity in milled fractions of brown rice, LWT – Food Sci. Technol. 88 (2018) 64 – 70.

I. Muraki, H.Wu, F. Imamura, F. Laden, E.B. Rimm, F.B. Hu, W.C. Willett, Q. Sun, Rice consumption and risk of cardiovascular disease: results from a pooled analysis of 3 U.S. cohorts, Am. J. Clin. Nutr. 101(1) (2015) 164 – 172.

V.S. Malik, V. Sudha, N.M. Wedick, M. RamyaBai. P. Vijayalakshmi, N. Lakshmipriya, R. Gayathri, A. Kokila, C. Jones, B. Hong, R. Li, K. Krishnaswamy, R.M. Anjana, D. Spiegelman, W.C. Willett, F.B. Hu, V. Mohan, Substituting brown rice for white rice on diabetes risk factors in India: a randomised controlled trial, Br. J. Nutr. 121(12) (2019) 1389 − 1397.

M.H. Payman, I. Bagheri, M.R. Alizadeh, R. Roohi, Effective parameters of broken rice during paddy hulling using rubber roll huller, J. Biol. Sci. 7 (2006) 45 − 71.

S. Kotupan, K. Sommart, Broken rice in a fermented total mixed ration improves carcass and marbling quality in fattened beef cattle, Anim Biosci. 34(8) (2021) 1331 − 134.

Y.D. Setyawati, S.F. Ahsan, L.K. Ong, F.E. Soetaredjo, S. Ismadji, Y.H. Ju, Production of glutinous rice flour from broken rice via ultrasonic assisted extraction of amylose, Food Chem. 15(203) (2016) 158 – 164.

L.V. Nunes, F.F. de Barros Correa, P. de Oliva Neto, C.R. Mayer, B. Escaramboni, T.S. Campioni, N.R. de Barros, R.D. Herculano, E.G. Fernández Núñez, Lactic acid production from submerged fermentation of broken rice using undefined mixed culture, World J Microbiol Biotechnol. 33(4) (2017) 79.

M. Chitpan, V. Chavasit, R. Kongkachuichai, Development of fortified dried broken rice as a complementary food, Food Nutr Bull. 26(4) (2005) 376 – 384.

S. Sriwattana, N. Laokuldilok, W. Prinyawiwatkul, Sensory Optimization of Broken‐Rice Based Snacks Fortified with Protein and Fiber, J. Food Sci. 73(6) (2008) 333 – 338.

L.C. Okpala, P.N. Egwu, Utilisation of broken rice and cocoyam flour blends in the production of biscuits, Niger. Food J. 33(1) (2015) 8 – 11.

H. Ti, R. Zhang, M. Zhang, Z. Wei, J. Chi, Y. Deng, Y. Zhang, Effect of extrusion on phytochemical profiles in milled fractions of black rice, Food Chem. 178 (2015) 186 – 194.

Y. Chen, Y. Ma, L. Dong, X. Jia, L. Liu, F. Huang, J. Chi, J. Xiao, M. Zhang, R. Zhang, Extrusion and fungal fermentation change the profile and antioxidant activity of free and bound phenolics in rice bran together with the phenolic bioaccessibility, LWT – Food Sci. Technol. 115 (2019) 108561.

M. Peanparkdee, C. Borompichaichartkul, S. Iwamoto, Bioaccessibility and antioxidant activity of phenolic acids, flavonoids, and anthocyanins of encapsulated Thai rice bran extracts during in vitro gastrointestinal digestion, Food Chem. 361 (2021) 130161.

M.J. Chu, X.M. Liu, N. Yan, F.Z. Wang, Y.M. Du, Z.F. Zhang, Partial purification, identification, and quantitation of antioxidants from wild rice (Zizania latifolia), Molecules. 23(2782) (2018) 1 – 16.

C. Chua, Y. Dua, X. Yua, J. Shic, X. Yuana, X. Liua, Y. Liua, H. Zhanga, Z. Zhanga, N. Yan, Dynamics of antioxidant activities, metabolites, phenolic acids, flavonoids and phenolic biosynthetic genes in germinating Chinese wild rice (Zizania latifolia), Food Chem. 318 (2020) 126483.

J. Khunchalee, P. Charoenboon, Study of free radical scavenging, total phenolic contents and tyrosinase inhibition activity of crude extract from Carissa carandas Linn, SNRU. J. Sci. Tech. 11(1) (2019) 26 – 34.

T. Hobanthad, S. Maneetong, Simple extraction for the scanning of antioxidant activity of vegetables and fruits in Buriram, Thailand by DPPH, ABTS and FRAP assays, SNRU. J. Sci. Tech. 11(3) (2019) 114 – 121.

J. Jandaruang, S. Preecharram, Determination of biochemical compo- sitions and antioxidant activities of Hom Mali 105 rice, SNRU. J. Sci. Tech. 12(1) (2020) 146 – 154.

A. Wongklom, P. Moonsin, K. Chantharasopon, Antioxidant activities and exhibit to stimulate immune cell production in the in vitro of Hibiscus sabdariffa Linn. and Phyllanthus emblica L. extracts, SNRU. J. Sci. Tech. 11(1) (2019) 18 – 25.

Y. Xu, Y. Jin, J. Su, N. Yang, X. Xu, Z. Jin, B. Cui, F. Wu, Changes in the nutritional value, flavor, and antioxidant activity of brown glutinous rice during fermentation, Food Biosci. 43 (2021) 101273.

H.Y. Seong, M. Kim, Enhanced protein quality and antioxidant activity of fermented Brown rice with Gryllus bimaculatus, LWT–Food Sci. Technol. 150 (2021) 111948.

M.A. Zubair, M.S. Rahman1, M.S. Islam1, M.Z. Abedin1, M.A. Sikder, A comparative study of the proximate composition of selected rice varieties in Tangail, Bangladesh, J. Environ. Sci. Nat. Resour. 8(2) (2015) 97 – 102.

A.O. Oko, B.E. Ubi, A.A. Efisue, N. Dambaba, Comparative analysis of the chemical nutrient composition of selected local and newly introduced rice varieties grown in Ebonyi State of Nigeria, Int. J. Agric. For. 2(2) (2012) 16 – 23

E. Vunain, F. Chirambo, S. Sajidu, T.T. Mguntha, Proximate composition, mineral composition and phytic acid in three common Malawian white rice grains, MJST. 12(1) (2020) 87 – 108.

D.K. Verma, P.P. Srivastav, Proximate composition, mineral content, and fatty acids analyses of aromatic and non–aromatic Indian rice, Rice Sci. 24(1) (2017) 21 – 31.

Z.K. Akalu, S.H. Geleta, Comparative analysis on the proximate composition of tubers of Colocasia Esculenta, L. Schott and Dioscorea alata cultivated in Ethiopia, Am. J. Biosci. Bioieng. 7(6) (2019) 93 – 101.

P.C. Eze, Determination of the proximate composition and amylose content of new rice for Africa (NERICA) flour, Turk. J. Agr. Eng. Res. 1 (2020) 131 – 140.

P. Hansakul, U. Srisawan, A. Itharat, N. Lerdvathisopon, Phenolic and flavonoid contents of Thai rice extracts and their correlation with antioxidant activities using chemical and cell assays, J. Med. Assoc. Thai. 94(7) (2011) 122 – 130.

V. Teeranachaideekul, A. Wongrakpanich, J. Leanpolchareanchai, K. Thirapanmethee, C. Sirichaovanichkarn, Characterization, biological activities, and safety evaluation of different varieties of Thai pigmented rice extracts for cosmetic applications, Pharm. Sci Asia. 45(3) (2018) 140 – 153.

P. Maisuthisakul, L. Changchub, Effect of extraction on phenolic antioxidant of difference Thai rice (Oryza Sativa L.) genotypes, Int. J. Food Prop. 17 (2014) 855 – 865.

P. Sanwiriyamongkol, P. Srisuk, Determination of phenolic compounds, proteins and antioxidant activity in rice water fraction extracted from Phayaleumkang grutinous rice, IJPS. 17(1) (2021) 68 – 79.

N. Wongpriaw, Determination of protein, mineral, and vitamin in local rice varieties from Loei province, RMUTP Res. J. 12(2) (2018) 158 – 171.

A. Dubock, Golden rice: to combat vitamin a deficiency for public health, Intechopen. (2019) 1 – 21.

S. Chooklin, T. Muadsri, P. Purintrapibal, Nutritional values of Trang indigenous brown rice, RMUTSV Res. J. 12(2) (2020) 285 – 296.




How to Cite

Suwannatrai, K., Namwongsa, K., Phanomkhet, N., Nuntapanich, H., & Roschat, W. (2022). The analysis of nutritional value, total phenolic and flavonoid contents, and antioxidant activities from the ethanolic extracts of the roasted broken brown rice powder. Creative Science, 14(2), 246426.