Cytogenetics of Black-bearded Tomb bat (Taphozous melanopogon) by Conventional staining, Ag-NOR staining and Fluorescence in situ hybridization Techniques

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Sucheela Talumphai
Sutheemon Techa-ay
Sukhonthip Ditcharoen
Alongkload Tanomthong

Abstract

Black-bearded Tomb bat is classified as the genus of Taphozous and has a very similar morphology. The knowledge can provide cytogenetic information potentially useful to support on taxonomic classification. Five males and six females were collected from Mahasarakam province and Khon Kaen university, Khon Kaen province. The 20% Giemsa’s and 50% silver nitrate solution, respectively were applied to stain the chromosomes. Chromosomes were prepared from bone marrow tissue. The chromosomes harvesting was investigated by hypotonic-fixation-air drying technique. Giemsa’s staining, Ag-NOR staining and fluorescence in situ hybridization (FISH) staining techniques, as well as microsatellites (GC)15 and (CGG)10 as probes were applied. The karyotype, idiogram, position of NORs and microsatellites were performed in the present study. The results show that the diploid chromosomes number was 2n = 42 and the fundamental number (NF) were 74 chromosomes in both female and male of T. melanopogon. The autosomes consisting of 8 large metacentric, 8 medium metacentric, 2 medium submetacentric, 2 medium acrocentric, 2 small metacentric, 4 small submetacentric, 4 small acrocentric and 10 small telocentric chromosomes. The sex determination is XY, X chromosome is medium metacentric chromosomes and Y chromosome is small submetacentric chromosomes. NOR sites appeared to telomere of the short arm of the chromosome pairs 15 small acrocentric type and chromosome pairs 20 small telocentric type. The pattern of both microsatellite (GC)15 and (CGG)10 repeats were distributed throughout the genome as well as to all chromosomes. Karyotype formula described as 2n (42) = Lm8 + Mm8 + Msm2 + Ma2 + Sm2 + Ssm4+ Sa4 + St10 + Sex Chromosome.

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1.
Talumphai S, Techa-ay S, Ditcharoen S, Tanomthong A. Cytogenetics of Black-bearded Tomb bat (Taphozous melanopogon) by Conventional staining, Ag-NOR staining and Fluorescence in situ hybridization Techniques. J Appl Res Sci Tech [Internet]. 2021 Sep. 27 [cited 2024 Jun. 18];20(2):57-69. Available from: https://ph01.tci-thaijo.org/index.php/rmutt-journal/article/view/244149
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Research Articles

References

Thongchai N, Kasidis R, Sakarin S, Art-ong P. Biodiversity of balts and small mammals in the secondary forest, RSPG area. Research Results and Research Progress Report; 2015.

Sotero-Caio CG, Baker RJ, Volleth M. Chromosomal evolution in Chiroptera. Genes. 2017;8(10):1-25.

Praween S, Alongklod T, Sumpars K, Krit P, La-orsri S. Karyological analysis and morphometrics of the Lesser Asiatic House Bat, Scotophilus kuhlii (Chiroptera, Vespertilionidae). Cytologia. 2012;77(3):401-11.

Praween S, Alongklod T, Sumpars K, Sarawut K, Krit P, La-orsri S. Karyological analysis and morphometrics of Horsfield's Bat, Myotis horsfieldii (Chiroptera, Vespertilionidae). Cytologia. 2013;78(1):15-24.

Sitthisak J, Praween S, Alongklod T, Sumpars K, Narumon Pr, Sarawut K. Chromosome analysis and morphometric of Intermediate Roundleaf Bat, Hipposideros larvatus (Chiroptera, Hipposideridae) by conventional, GTG-banding and Ag-NOR banding techniques. Cytologia. 2014;79(4):445-56.

Asan N, Albayrak I. Some taxonomic features of Taphozous nudiventris Cretzschmar, 1830 vel 1831 from Turkey (Chiroptera: Emballonuridae). Turk J Zool. 2007;12(31):165-70.

Liang-Kong L, Masaharu M, Masashi H. Karyology of Ten Vespertilionid Bats (Chiroptera: Vespertilionidae) from Taiwan. Zool Stud. 2002;41(4):347-54.

Schulz-Schaeffe J. Cytogenetics: Plants, Animals, Humans. New York: Springer-Verlag New York Inc.; 1980.

Almeida B, Novaes RLM, Aguieiras M, Souza RF, Esbérard CEL, Geise L. Karyotype of three Lonchophylla species (Chiroptera, Phyllostomidae) from Southeastern Brazil. Comp Cytogenet. 2016;10(1):109-15.

Nuntiya M, Weerayuth S, Alongklod T. Chromosomal analysis and Nors polymorphism of bagarius suchus (Siluriformes: Sisoridae) by conventional banding and fish techniques. J Nat Appl Sci. 2016;2(1):10-9.

Gálvez F, Symonová R, Kovařík A. Evolutionary trends in animal ribosomal DNA loci: Introduction to a new online database. Chromosoma. 2018;127:141-50.

Supiwong W, Tanomtong A, Pinthong K, Kaewmad P, Poungnak P, Jangsuwan N. The first chromosomal characteristics of nucleolar organizer regions and karyological analysis of pink anemonefish, Amphiprion perideraion (Perciformes, Amphiprioninae). Cytologia. 2015;80(3):271-8.

Kasiroek W, Indananda C, Luangoon N, Pinthong K, Supiwong W, Tanomtong A. First chromosome analysis of the Humpback cardinalfish, Fibramia lateralis (Perciformes, Apogonidae). Cytologia, 2017;82(1):9-15.

Tanomtong A, Supiwong W, Jearranaiprepame P, Khakhong S, Kongpironchuen C, Getlekha N. A new natural autotetraploid and chromosomal characteristics of dwarf snakehead fish, Channa gachua (Perciformes, Channidae) in Thailand. Cytologia. 2014;79(1):15-27.

Turpin R, Lejeune J. Les chromosomes humans. Paris: Gauthier-Pillars; 1965.

Nash WG, OíBrien SJ. A comparative chromo-some banding analysis of Ursidae and their relation-ship to other carnivores. Cytogenet Cell Genet. 1987;45:206-12.

Wada MY, Lim Y, Wurster-Hill DH. Bandedkaryotype of wild-caught male Korean raccoon dog, Nyctereutes procyonoides koreensis. Genomics. 1991;34:302-6.

Rodrigues DS, Rivera M, Lourenço LB. Molecular organization and chromosomal localization of 5S rDNA in Amazonian Engystomops (Anura, Leiuperidae). BMC Genet. 2012;13(1):1-13.

Koubínová D, Sreepada K, Koubek P, Zima J. Karyotypic variation in rhinolophid and hipposiderid bats (Chiroptera: Rhinolophidae, Hipposideridae) Acta Chiropt. 2010;12:393–400.

Seim I, Fang X, Xiong Z, Lobanov AV, Huang Z, Ma S, et al. Genome analysis reveals insights into physiology and longevity of the Brandt’s bat Myotis brandtii. Nat. Commun. 2013;4:1–8.

Rickart AE, Mercier AJ, Heaney RL. Cytogeography of philippine bat (Mammalia: Chrioptera). Biological society of Washington. 1999;112(3):453-69.

Asan N, Albayrak I. Some taxonomic features of taphozous nudiventris cretzschmar, 1830 vel 1831 from Turkey (Chiroptera: Emballonuridae). Turk J Zool. 2007;12(31):165-70.

Harada M, Minezawa M, Takada S, Yenbutra S, Nunpakdee P, Ohtani S. Karyological analysis of 12 species of bats from Thailand. Caryologia. 1982;352:269-78.

Sotero-Caio CG, Baker RJ, Volleth M. Chromosomal Evolution in Chiroptera. Genes. 2017;8(10):1-25.

Ray-Chaudhuri SP, Pathak S, Sharma T. Karyotypes of five Indian species of microchiroptera. Research project. Department of Zoology, Banaras Hindu University. Caryologia. 1971;24(2):239-45.

Arsalan A, Zima J. Karyotypes of the mammals of Turkey and neighbouring regions: A review. Folia Zool. 2014;63(1):1–62.