ENHANCING LONGEVITY AND BACTERIAL VIABILITY OF VEGETABLE SOYBEAN SEEDS THROUGH BIO-COATING WITH ENTEROBACTER KOBEI

ผู้แต่ง

  • Sukanya Baomeesri Division of Agronomy, Faculty of Agricultural Production, Maejo University
  • Jiraporn Inthasan Bachelor of science (Soil Resources and Environment), Faculty of Agricultural Production, Maejo University
  • Vassana Viroonrat Bachelor of science (Soil Resources and Environment), Faculty of Agricultural Production, Maejo University
  • Jakkrapong Kangsopa Division of Agronomy, Faculty of Agricultural Production, Maejo University; Modern Seed Technology Research Center, Faculty of Agricultural Production, Maejo University

คำสำคัญ:

Seed enhancement, Plant growth-promoting rhizobacteria, Seed storage, Bio-polymer

บทคัดย่อ

Vegetable soybean is valued for its high nutritional content and growing consumer demand. However, its cultivation often faces challenges related to poor seed germination, low seedling vigor, and limited storability. This study aimed to evaluate the potential of Enterobacter kobei as a bio-coating agent to enhance seed quality before and after storage. Seeds were coated with E. kobei at five different concentrations (103, 104, 105, 106, and 107 CFU/mL) and evaluated under two germination test conditions: sand and top of paper covered with sand. Results showed that coating seeds with E. kobei at 105–107 CFU/mL significantly improved germination percentage, speed of germination, and reduced mean germination time; MGT compared to uncoated seeds and those coated with polymer alone. The three effective treatments (105, 106 and 107 CFU/mL) were subsequently subjected to storage under controlled and ambient conditions. After six months of controlled storage, seeds coated with E. kobei at 106 CFU/mL maintained high germination rates and consistent seedling shoot length, indicating stable performance. In contrast, seeds stored under ambient conditions showed a marked decline in quality after four months, particularly in germination and seedling growth. Additionally, bacterial viability was found to be influenced by inoculum density, with 106 CFU/mL providing the optimal balance between viable microbial population and long-term survival. These findings suggest that E. kobei at 106 CFU/mL is an effective bio-coating agent for improving seed performance and storability in vegetable soybean.

เอกสารอ้างอิง

Accinelli, C., Abbas, H.K., Little, N.S., Kotowicz, J.K., & Shier, W.T. (2018). Biological control of aflatoxin production in corn using non-aflatoxigenic Aspergillus flavus administered as a bioplastic-based seed coating. Crop Protection, 107, 87-92.

AOSA. (1983). Seed vigor testing handbook (Contribution No. 32 to the Handbook on Seed Testing). Lincoln, NE: AOSA.

Bailly, C., Leymarie, J., Lehner, A., Rousseau, S., Côme, D., & Corbineau, F. (2004). Catalase activity and expression in developing sunflower seeds as related to drying. Journal of Experimental Botany, 55(396), 475-483.

Bewley, J.D., Bradford, K.J., Hilhorst, H. W. M., & Nonogaki, H. (2013). Seeds: Physiology of development, germination and dormancy. (3rd edition). Springer.

Chadha, M.L., & Oluoch, M.O. (2004). Vegetable soybean research and development in Africa (pp. 921-928). In C. Masuda & M. L. Chadha (Eds.), Proceedings of the International Workshop on Vegetable Soybean Research and Development. Shanhua, Taiwan: Asian Vegetable Research and Development Center (AVRDC).

Copeland, L.O., & McDonald, M.B. (2001). Seed Enhancements. In: Principles of Seed Science and Technology. Boston: Springer.

Deaker, R., Roughley, R.J., & Kennedy, I.R. (2007). Desiccation tolerance of rhizobia when protected by synthetic polymers. Soil Biology and Biochemistry, 39(2), 573-580.

Egamberdieva, D., Wirth, S.J., Alqarawi, A.A., Abd-Allah, E.F., & Hashem, A. (2017). Phytohormones and beneficial microbes: essential components for plants to balance stress and fitness. Frontiers in Microbiology, 8, 2104.

Ellis, R.H., & Roberts, E.H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9(2), 373–409.

Gemell, L.G., Hartley, E.J., & Herridge, D.F. (2005). Point-of-sale evaluation of preinoculated and custom-inoculated pasture legume seed. Australian Journal of Experimental Agriculture, 45(3), 161-169.

Ghosh, P.K., Sen, S.K., & Maiti, T.K. (2015). Production and metabolism of IAA by Enterobacter spp. (Gammaproteobacteria) isolated from root nodules of a legume Abrus precatorius L. Biocatalysis and Agricultural Biotechnology, 4(3), 296-303.

Glick, B.R. (2012). Plant growth‐promoting bacteria: mechanisms and applications. Scientifica, 2012(1), 963401.

Halmer, P. (2000). Commercial seed treatment technology. Seed Technology and Its Biological Basis. England: Sheffield Academic Press.

ISTA. (2024). International Rules for Seed Testing, Edition 2024. Bassersdorf: International Seed Testing Association.

Kangsopa, J., Singsopa, A., & Thawong, N. (2023). Effects of different binder types and concentrations on physical and quality properties in marigold (Tagetes erecta L.) seed pelleting. Songklanakarin Journal of Science and Technology, 45(4), 494–500.

Kumar, N., & Dubey, R.C. (2022). Plant growth promoting endophytic bacteria Bacillus australimaris BLR41 and Enterobacter kobei BLR45 enhance the growth of medicinal plant Barleria lupulina Lindl. Journal of Pure & Applied Microbiology, 16(4), 2647-2658.

Ma, Y. (2019). Seed coating with beneficial microorganisms for precision agriculture. Biotechnology Advances, 37(7), 107423.

Mardmai, S., Banthoengsuk, S., & Fakthongphan, J. (2023). Effect of seed priming with KNO3 on germination and vigor of Chiang Mai 84-2 vegetable soybean seed. Journal of Science and Technology, 12(3), 92-104.

McDonald, M.B. (1999). Seed deterioration: Physiology, repair and assessment. Seed Science and Technology, 27(1), 177–237.

Mnasri, N., Chennaoui, C., Gargouri, S., Mhamdi, R., Hessini, K., Elkahoui, S., & Djébali, N. (2017). Efficacy of some rhizospheric and endophytic bacteria in vitro and as seed coating for the control of Fusarium culmorum infecting durum wheat in Tunisia. European Journal of Plant Pathology, 147, 501-515.

Nair, R.M., Boddepalli, V.N., Yan, M.R., Kumar, V., Gill, B., Pan, R.S., & Somta, P. (2023). Global status of vegetable soybean. Plants, 12(3), 609.

Ortega-Ortega, Y., Sarmiento-López, L.G., Baylón-Palomino, A., Vázquez-Lee, J., Maldonado-Bonilla, L.D., Flores-Olivas, A., & Valenzuela-Soto, J.H. (2024). Enterobacter sp. DBA51 produces ACC deaminase and promotes the growth of tomato (Solanum lycopersicum L.) and tobacco (Nicotiana tabacum L.) plants under greenhouse condition. Current Research in Microbial Sciences, 6, 100207.

Paravar, A., Piri, R., Balouchi, H., & Ma, Y. (2023). Microbial seed coating: an attractive tool for sustainable agriculture. Biotechnology Reports, 37, e00781.

Park, J.M., Radhakrishnan, R., Kang, S.M., & Lee, I.J. (2015). IAA producing Enterobacter sp. I-3 as a potent bio-herbicide candidate for weed control: a special reference with lettuce growth inhibition. Indian journal of Microbiology, 55, 207-212.

Pedrini, S., Merritt, D.J., Stevens, J., & Dixon, K. (2017). Seed coating: science or marketing spin?. Trends in Plant Science, 22(2), 106-116.

Qiu, Y., Amirkhani, M., Mayton, H., Chen, Z., & Taylor, A.G. (2020). Biostimulant seed coating treatments to improve cover crop germination and seedling growth. Agronomy, 10(2), 154.

Rahman, M.S., Hasan, M.S., Nitai, A.S., Nam, S., Karmakar, A.K., Ahsan, M.S., & Ahmed, M.B. (2021). Recent developments of carboxymethyl cellulose. Polymers, 13(8), 1345.

Rocha, I., Ma, Y., Souza-Alonso, P., Vosátka, M., Freitas, H., & Oliveira, R.S. (2019). Seed coating: a tool for delivering beneficial microbes to agricultural crops. Frontiers in Plant Science, 10, 1357.

Sahu, T., Choudhary, R., & Kulkarni, P. (2025). Optimization of cellulase production by Enterobacter quasihormaechei using pressmud waste as substrate. Ecology, Environment & Conservation (0971765X), 31, 423-429.

Syaziana, N.S.A., Othman, N.M.I., Soraya, S.A., Ali, T.K.Z., & Musliyana, M. (2024). Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth. Journal of King Saud University-Science, 36(6), 103197.

Taylor, A.G., Allen, P.S., Bennett, M.A., Bradford, K.J., Burris, J.S., & Misra, M.K. (1998). Seed enhancements. Seed Science Research, 8(2), 245-256.

Thomma, K., & Sirithorn, P. (2012). Effect of antagonistic bacterium Bacillus subtilis B006 as seed coating for control of Botryosphaeria rhodina, cause of gummosis disease in cucurbit. Khon Kaen Agriculture Journal, 40, 53-60.

Venturi, V., & Keel, C. (2016). Signaling in the rhizosphere. Trends in Plant Science, 21(3), 187-198.

ดาวน์โหลด

เผยแพร่แล้ว

2026-08-28

รูปแบบการอ้างอิง

Baomeesri, S., Inthasan, J., Viroonrat, V., & Kangsopa, J. (2026). ENHANCING LONGEVITY AND BACTERIAL VIABILITY OF VEGETABLE SOYBEAN SEEDS THROUGH BIO-COATING WITH ENTEROBACTER KOBEI. PSRU Journal of Science and Technology, 11(2), 135–153. สืบค้น จาก https://ph01.tci-thaijo.org/index.php/Scipsru/article/view/262803

ฉบับ

ปีที่ 11 ฉบับที่ 2 (2026): May - August 2026

ประเภทบทความ

บทความวิจัย

สัญญาอนุญาต

ลิขสิทธิ์ (c) 2026 PSRU Journal of Science and Technology

Creative Commons License

อนุญาตภายใต้เงื่อนไข Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

กองบรรณาธิการขอสงวนสิทธิ์ในการปรับปรุงแก้ไขตัวอักษรและคำสะกดต่างๆ ที่ไม่ถูกต้อง และต้นฉบับที่ได้รับการตีพิมพ์ในวารสาร PSRU Journal of Science and Technology ถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม และ
ผลการพิจารณาคัดเลือกบทความตีพิมพ์ในวารสารให้ถือมติของกองบรรณาธิการเป็นที่สิ้นสุด