PSRU Journal of Science and Technology <p>&nbsp; &nbsp; &nbsp; &nbsp; PSRU Journal of Science and Technology is a peer-reviewed journal of Faculty of Science and Technology, Pibulsongkram Rajabhat University. The journal devoted to publish of&nbsp; original research article and review article in science and technology. The journal is indexed in TCI Tier 2.</p> <p>Three issues are published a year (January-April, June-August and September-December).</p> <p>Online ISSN 2672-9741</p> <p>Print ISSN 2465-5112</p> คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม en-US PSRU Journal of Science and Technology 2465-5112 <p>กองบรรณาธิการขอสงวนสิทธิ์ในการปรับปรุงแก้ไขตัวอักษรและคำสะกดต่างๆ ที่ไม่ถูกต้อง และต้นฉบับที่ได้รับการตีพิมพ์ในวารสาร PSRU Journal of Science and Technology ถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม และ<br>ผลการพิจารณาคัดเลือกบทความตีพิมพ์ในวารสารให้ถือมติของกองบรรณาธิการเป็นที่สิ้นสุด&nbsp;</p> THE PRELIMINARY INVESTIGATION OF USING WASTE PLASTER GYPSUM MOLD AS SAND REPLACEMENT MATERIAL IN MORTAR <p>This research aims to study the possibility of using waste plaster gypsum mold from <br />the ceramic industry in Lampang Province to produce wall materials. Waste plaster gypsum mold was ground and sieved to keep similar particle size distribution to those of sand. The ground plaster gypsum was then replaced with sand in the mortar at 10, 30, 50, 60, 80, 90, and 100 percent by volume. The effect of waste plaster gypsum on physical, mechanical, and thermal properties was carried out. The results showed that compressive strength, tensile strength and flexural strength were decreased with the increasing amount of waste plaster gypsum. The compressive strength of mortars with the replacement of waste plaster gypsum at 50 and 100 percent by volume at 28 days was 10.50 and 7.00 Megapascal, respectively. However, the thermal conductivity of mortar containing 100 percent waste plaster gypsum was 0.38 Watts per meter per Kelvin, which was reduced by 78 percent compared to that of the control mortar. It achieved a density approximately at 1.60 gram per cubic centimeter. Therefore, waste plaster gypsum as sand replacement material might result in a lightweight wall block with suitable thermal insulation properties. </p> Pincha Torkittikul Thachon Kaosomboon Watthana Makararotrit Sarayut Malai Apiwat Sripuman Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 1 13 DEVELOPMENT OF A SIMPLE COLORIMETRIC SENSOR FOR THE DETECTION OF COPPER AND IRON METAL IONS <p>This research was to develop a micro-scale sensor for Cu and Fe metal ions determination by using extracted anthocyanins in butterfly pea (<em>Clitoria ternatea</em> L.) which is used as a natural indicator based on the interaction between anthocyanin and metal ions to form a complex compound, caused a change in the absorbance by naked-eye detected color change. From the results, butterfly pea extracted had maintained the stability of absorption spectra by water solvent more than alcohol solvent. Anthocyanin extracted from butterfly pea reacted with Cu and Fe metal ions showed the most clearly change in color under 0.01% acetate buffer at pH 6 due to the number of protons increased in anthocyanin structure. As <br />a result, the absorbance was shifted to the lower wavelength and the color changed from dark blue to purple since the flavylium cation was formed. The addition of divalent metal ion group except carbonate ion showed small interference with the color of detection. The quantitative analysis results by using the solution sensor system agreed well with the atomic absorption spectrometry (AAS) analysis. In addition, the optimum conditions obtained from the solution system were applied to a paper-based sensor coated on the filter paper. It was found that the results of Cu and Fe metals ions analysis were consistent with the solution sensor with naked-eye detection. Moreover, the developed Cu and Fe metal ions sensor would save time, cost-effectiveness and environmental friendliness.</p> Kaewalee Chobtam Chonlada Dechakiatkrai, Theerakarunwong Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 14 29 GENETIC DIVERSITY OF SOME SPECIES IN GENUS GLOBBA AT SAM PHRAO CAMPUS UDON THANI RAJABHAT UNIVERSITY BY USING RAPD MARKERS <p>This research was to study the genetic diversity of some plants in the genus <em>Globba</em> by using RAPD markers combined with basic plant morphology. The morphological characters of the nine accessions were obtained DNA extraction by using the modified CTAB technique from Doyle &amp; Doyle (1987). The DNA amplification was executed by using a PCR RAPD technique and analyzed the genetic association with UPGMA method. The result showed that the DNA could be amplified with 4 of 25 primers includes OPA-03, OPC-05, OPC-06 and OPX-12 primers. Total 141 DNA bands could be detected with size approximately 250-2500 base pair. Moreover, the phylogenetic analysis shown an interval 0.420-0.938 and resulting in plant categorized into two groups. These are correlated with the plant morphology, by the staminode and appendages characters. The first group has a staminode extending off the floral tube and has two pairs of appendages, <em>Globba siamensis</em> (Hemsl.) Hemsl. The second group has a staminode clinging to the floral tube and has with or without two pairs of appendages, <em>Globba laeta</em> K. Larsen, <em>Globba</em> sp., and <em>Curcuma angustifolia </em>Roxb. However, the RAPD marker is useful for classifying plants within the genus <em>Globba</em> and closely related other genera.</p> Tasanai Punjansing Wanlaya Mongkolsawat Sukanda Boonraksa Viboon Pensuk Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 30 41 THE FIBER EXTRACTION AND THE STUDY OF PROPERTIES OF SUGAR PALM (ARENGA PINNATA WERR) PEDUNCLE'S FIBER <p>The purposes of this research were to study the process of the fiber extraction and study the properties of Sugar Palm peduncle's fiber. Fiber extraction was investigated into <br />3 experimental sets which used sodium hydroxide solution at concentrations of 5%, 10%, and 15%w/v respectively. Each experimental set takes boiling time for 3 hours. The study revealed that it is most effective for extracting Sugar Palm peduncle's fiber to use a 10%w/v sodium hydroxide solution with the yield of fiber 43.21<u>+</u>0.30%, cellulose 41.71<u>+</u>0.35% hemicellulose 18.49<u>+</u>0.98%, lignin 2.94<u>+</u>0.07%, yarn size (Ne) 8.65<u>+</u>0.05, tensile strength (gram-force) 1,256.32<u>+</u>43.75 and elongation at break point 17.75<u>+</u>0.43%. The peduncle fibers are 50 µm in diameter. The Sugar Palm peduncle’s fiber is characterized by long fiber, rough surface and large size. Therefore, Sugar Palm peduncle’s fibers are suitable to be used in the development of decorative or decorative textile products to create a unique identity for community products rather than being used to develop into apparel products.</p> Tawatchai Jitwarin Boonchat Mekkaeo Kanyapak Nantachai Benjakarn Nirattisai Pattarawat Sriboonjit Sufianee Sani Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 42 56 SCREENING LACTIC ACID BACTERIA FROM LOCAL FERMENTED FOOD FOR USING AS PROBIOTIC IN AQUATIC ANIMAL FEED MIXED WITH DIETARY FIBER FROM DURIAN RIND <p>This research aimed to use dietary fiber (DF) from durian rind as a prebiotic with probiotic bacteria from fermented food in the aquatic feed for feeding the Thai carp. <br />The experiments comprised of screening the lactic acid bacteria (LAB) from fermented food and preparation of aquatic animal feed by using DF from durian rind as a prebiotic with the isolated probiotic bacteria for feeding the Thai carp. The results of screening the LAB from 30 fermented food samples found that 35 isolates of LAB were isolated and all isolate were able to grow in anaerobic condition. There were 31 LAB isolates could grow in aerobic and anaerobic conditions. For the probiotic properties, there were 6 LAB isolates could tolerate acid and bile salt conditions. The LAB with the best probiotic property and growth was selected to use in the next experiment. The preparation diet was started by preparing the DF from durian rind, mixed the DF in Thai carp diet at the percentage of 0, 10, 20, 30, and 40 mixed the obtained diet with probiotic cells and used diet to feed fishes. The results found that <br />the utilization of DF from durian rind mixed in Thai carp diet at different levels led to <br />the growth of trial fish included weight gain, average daily gain (ADG), specific growth rate (SGR), feed conversion ratio and survival rate were not significant difference from the control. From the results indicated that the aquatic animal prepared from DF of durian rind could be used with isolated probiotic bacteria from fermented food for feeding Thai carp and can be a guideline to add value of durian rind.</p> <p> </p> Yardrung Suwannarat Punyisa Wattanachai Sarawut Sangsawanchote Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 57 72 SOIL QUALITY ASSESSMENT IN POMELO ORCHARDS IN PHO PRATHAP CHANG SUB-DISTRICT, PHO PRATHAP CHANG DISTRICT, PHICHIT PROVINCE <p>This research was to study the soil quality of three pomelo varieties’s orchards including Thakhoi, Kaotangkwa and Thongdee varieties in Pho Prathap Chang Sub-district, Pho Prathap Chang District, Phichit province. Soil samples were randomly collected in 10 orchards of each pomelo variety, a total of 30 orchards. The results showed that soil color of pomelo orchards was light brown to dark brown. The soil textures were mostly silt loam. Soil pH was slightly acidic with an average of 6.23±0.61. The average moisture was 8.16±3.81%. Soil organic matters were at moderately high to high levels with an average of 3.39±0.86%. Nitrogen contents were at a moderate level with an average of 0.17±0.04%. The amounts of phosphorus were at a low level with an average of 7.48±4.60 mg/kg, and potassium contents were at <br />a moderate level with an average of 86.24±9.31. Soil organic matter and potassium contents in the soil of the pomelo orchards were considered suitable for pomelo cultivation, while nitrogen and phosphorus contents were lower than the suitable criteria for growing pomelo. Therefore, farmers should regularly determine nitrogen and phosphorus contents in pomelo’s orchard soils to be basic information of nutrient management in pomelo’s orchard soils properly. This will enable the soils to have the potential to further increase the pomelo productivity and quality.</p> Parichat Thipphawan Piyada Wachirawongsakorn Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 73 88 STUDY OF COLOR HUES AND FASTNESS OF NATURAL DYES FROM THE LEAVES OF BOUAE BURMANICA GRIFF. ON SILK AND COTTON <p>The objectives of this research were to study natural dyeing using dyes extracted from fresh and dried leaves of <em>Bouea burmanica</em> Griff, to identify color differences, and to evaluate the color fastness to light, washing, perspiration and abrasion. Four types of mordants, namely alum, lime water, iron and fresh eucalyptus leaves were used in the experiments and pre-mordant staining condition was used. The results showed that the dyes from fresh and dried leaves were able to dye silk and cotton fibers and obtained different shades according to the type of mordants, including yellow, brown and black. In both types of fibers, the dye from the fresh leaves produced a brighter color than the dye from the dried leaves. Silk fiber was dyed in a brighter hue than cotton fiber. The usage of iron as a mordant gave the least bright color. The dyes from fresh and dried leaves gave a brown hue in both silk and cotton fibers, except when using alum as a mordant the fresh leaves will give a yellow hue. In addition, when iron was used as a mordant, it gave a black hue. It was found that the dye from the fresh leaves gives a darker hue than the dye from the dried leaves. Color fastness to light, washing, perspiration and abrasion were all in acceptable levels. This research shows that the leaves of <em>B. burmanica </em>can be used as a new natural dye and can help to further enhance the development of valuable woven products.</p> Pattawat Seekhaw Nuengruethai Nearmayhom Naruemon Promla Nutsarin Sukonpakdee Kunchit na wichian Saowalak Yoopech Aranya Jutiviboonsuk Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 89 102 BIOLOGICAL ACTIVITIES, PHENOLIC AND VITAMIN C CONTENTS FROM SYZYGIUM CUMINI (L.) EXTRACT <p>Cosmeceutical products are more attractive nowadays. <em>Syzygium cumini</em> fruit, a Thai fruit possess wide variety of biological activities. However, its cosmeceutical property has not reported yet. The aims of this study are to study the antioxidant activity, antityrosinase activity, inhibition of acne causing bacteria, analysis of phenolic content and vitamin C from <em>Syzygium cumini</em> extract. The results indicated that<em> Syzygium cumini</em> extract showed antioxidant activity with IC<sub>50</sub> of 85.22±4.35 µg/ml. The phenolic content and Vitamin C of <em>Syzygium cumini</em> extract were 18.50±1.84 mg gallic acid equivalent/ g extract and 15.24±1.84 mg/g extract, respectively. Moreover, antityrosinase activity showed IC<sub>50</sub> to 181.59±4.77 µg/ml. Also, <em>Syzygium</em><em> cumini</em> extract in concentration of 50 mg/ml inhibited <em>Propionibacterium acnes</em> and <em>Staphylococcus aureus</em> to 1.45±0.07 cm and 0.95±0.50 cm, respectively. Therefore, <em>Syzygium cumini</em> extract can be further developed for cosmeceutical products. However, skin irritation should be evaluated.</p> Montra Srisayam Chawalit Puengtang Anong Srisopa Arunluk Chodnakarin Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 103 113 EFFECT OF LIGHT SPECTRUM ON CACTUS GERMINATION AND GROWTH <p>The purpose of this research was to study the effects of light spectra of LED light sources and sunlight with shading on germination and growth of Gymnocalycium and Astrophytum asterias cactus seeds. Cactus seeds were planted in a closed system with 10 seeds per pot under the light of a plant grow lamp, a lamp that produces white, green, blue, and red light. This included seeding experiments under sunlight and with 60% silver sunshade net of 1, 2, and 3 layers. The growth photographs were recorded for analysis to determine the diameter of the cactus. It was found that under the light from the plant-grow lamp and the white-light lamp, the germination rate of both cultivars was high, the growth was rapid in the first period and the growth slowed down in the latter. The green-light and blue-light lamps had low seed germination rates and slower growth. The red-light lamp had high germination rates but had abnormally elongated stem growth. The experiments planted under the sunlight without shading showed low germination rate and poor growth. However, germination and growth rates were higher when planted under 2 and 3 layers of sunshade net. From the research, <br />it was found that under the sunlight optimal conditions for the highest germination and growth are 2 layers of 60% silver sunshade net. It can be grown indoors using artificial LED lights which are used as both plants grow light and white light. It can be utilized in cacti planting to increase its value from high yield and beautiful stem quality.</p> Kachain Dangudom Yorkorn Wandee Wanvisa Chatpangta Parvinee Boonyaras Saengphet Boonpang Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 114 125 ANTIOXIDANT CAPACITY OF WOOD-DESTROYING FUNGI EXTRACTS <p>This research study aimed to evaluate the antioxidant activity of wood destroying fungi extracts. The 13 wood destroying fungi (NK0001, NK0049, NK0182, NK0317, NW1820, NW1821, NW1826, NW1836, NW1838, NW1842, NW1847, NW1850, and NW1851) obtained from the Center of Excellence in Microbial Diversity and Suitable Utilization, Chiang Mai University, Chiang Mai. All fungal isolates were cultured on Potato Dextrose Broth (PDB) for 14 days, then the mycelium was filtered and dried in hot air oven (45 °C). Dry mycelium was separately extracted by maceration with ethanol for 48 hours, filtered and adjusted to 10 mL with ethanol. The antioxidant properties of extracts based on 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity assay were analyzed. The result found that the wood destroying fungi extract (1000 ug/ml) could inhibit free radical among 5.47-90.06 %.The effective strains that indicated as high antioxidant activity (percent inhibition &gt;50) were NW1851 (90.06%), NK001 (78.86%), NW1836 (69.12%), NW1842 (67.48%), NW1826 (62.81%), and NW1820 (59.68%). The highest antioxidant activity of the six isolates was NW1851 (IC<sub>50</sub> value = 39.96 µg/ml). Furthermore, Total phenolic, total flavonoid and total tannin contents were also determined. The highest total phenolic and total tannin contents were found in the extract of NW1851 (2.17 mg GAE/g extract and 1.34 mg TAE/g extract, respectively). Whereas, the extract of NK0049 exhibited the highest content of flavonoid (1.20 mg QE/g extract). This research indicates that wood-destroying fungi have antioxidant activity that could be used to develop products in the food, pharmaceutical, and cosmetic industries in the future.</p> Wanpen Trongtorkit Chaowalit Phungtang Nopparat Wannathes Pisit Poolprasert Ruangwut Chutima Weera Nakphu Suthamat ๋๋Janjam Keerati Tanruean Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 126 137 บทบรรณาธิการ <p>บทบรรณาธิการ</p> Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2 สารบัญ <p>สารบัญ</p> Copyright (c) 2022 PSRU Journal of Science and Technology 2022-08-24 2022-08-24 7 2