ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS OF 7 THAI MEDICINAL PLANT EXTRACTS
Keywords:
Medicinal plants, S. aureus, AntibacterialAbstract
Staphylococcus aureus is a major human pathogen causing infections in multiple organs and is now becoming antibiotic-resistant which is a global crisis. The aim of this study was to investigate the antibacterial effect of medicinal plant extracts for S. aureus inhibition. Crude extracts of plants, including Psidium guajava L. leaf, Oroxylum indicum (L.) Kurz. leaf, Garcinia cowa Roxb. ex Choisy leaf, Curcuma longa L. rhizomes, Alpinia galanga (L.) Willd. rhizomes, Cinnamomum verum, and Syzygium aromaticum L., were obtained by ethanol extraction. Their antibacterial activities were investigated by disk diffusion method. The minimum inhibitory concentration; MIC was performed by the broth microdilution method and the minimum bactericidal concentration; MBC values of these extracts was tested. The results found that 4 herb extracts namely P. guajava leaf, G. cowa leaf, A. galanga rhizomes and S. aromaticum rhizomes showed an inhibitory activity which the inhibition zone as 9.54-22.93 millimeters. The MIC and MBC values of these extracts were in the range of 0.25-8 mg/ml and 2-128 mg/ml, respectively. The G. cowa leaves extract was the best antibacterial activity which MIC and MBC values of 0.2 and 2 mg/ml, respectively. In addition, all extracts showed the MBC to MIC ratio value greater than 4. Therefore, these extracts presented the bacteriostatic activity. These results show that many Thai herbs are effective in inhibiting pathogens. It can be developed into an antimicrobial product used in food, which can help to reduce the incidence of food-borne diseases.
References
ณัฏฐิณี ธีรกุลกิติพงศ์, อาลักษณ์ ทิพยรัตน์, ภักดี สุขพรสวรรค์, อาณัติ ดีพัฒนา, สมชาติ โชคชัยธรรม, จักริน สุขสวัสดิ์ชน, และมารุต ตั้งวัฒนาชลีพร. (2562). การวิเคราะห์ความไวต่อยาปฏิชีวนะรูปแบบใหม่และมีประสิทธิภาพสูง สำหรับการทดสอบตัวอย่างทางคลินิกระดับจุลภาคด้วยวิธีการพิสูจน์เอกลักษณ์ภาพดิจิตอล (รายงานการวิจัย). ชลบุรี: มหาวิทยลาลัยบูรพา.
ธนวัฒน์ ทองจีน, อัศวชัย ช่วยพรหม, สายัณห์ เรืองเขตร, และศักดิ์วิชัย อ่อนทอง. (2565). การตรวจสอบคุณสมบัติทางกายภาพและทางเคมีของใบชะมวง. วารสารการแพทย์แผนไทยและการแพทย์ทางเลือก, 20(3), 536-550.
สุกัญญา จันทร์สุนะ, ลลิตา เจริญทรัพย์, เยาวพา จิระเกียรติกุล, และพรชัย หาระโคตร. (2563). ผลของอุณหภูมิ และระยะเวลาการอบแห้งต่อปริมาณสารต้านอนุมูลอิสระและความสามารถในการยับยั้งอนุมูลอิสระของใบบัวบก [Centella asiatica (L.) Urb.]. วารสารวิทยาศาสตร์และเทคโนโลยี, 28(12), 2261-2272.
สุวรรณดี ทรัพย์เจริญ, และณัฐชา ด้วงรัก. (2567). ฤทธิ์ยับยั้งแบคทีเรียก่อโรคทางเดินอาหารของสารสกัดเอทานอลขมิ้นชัน. วารสารกรมวิทยาศาสตร์การแพทย์, 66(1), 18-33.
Anosa, G.N., Ezenduka, E.V., Gabriel, K.C., Ngene, A., & Eze, U.U. (2019). Occurrence and antibiogram of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus from table eggs in Nsukka, Enugu State, Nigeria. Sokoto Journal of Veterinary Science, 17(1), 62-70.
Ayoola, G.A., Coker, H.A.B., Adesegun, S.A., Adepoju-Bello, A.A., Obaweya, K., Ezennia1, E.C., & Atangbayila, T.O. (2008). Phytochemical Screening and Anti-oxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7(3), 1019-1024.
Aziz, I.M., Alfuraydi, A.A., Almarfadi, O.M., Aboul-Soud, M.A.M., Alshememry, A.K., Alsaleh, A.N., & Almajhdi, F.N. (2024). Phytochemical analysis, antioxidant, anticancer, and antibacterial potential of Alpinia galanga (L.) rhizome. Heliyon, 10(17), 1-16.
Bashir, A., Ado, A., & Alli, A.I. (2021). Determination of Antibacterial Activity of Psidium guajava Leaf Extract against Bacteria Isolated from Mobile Phones of Umaru Musa Yar’adua University, Katsina Community. UMYU Journal of Microbiology Research, 6(1), 219-226.
Bernardini, S., Tiezzi, A., Masci, V.L., & Ovidi, E. (2018). Natural products for human health: an historical overview of the drug discovery approaches. Natural Product Research, 32(16), 1926–1950.
Biology LibreTexts. (2023). 7.1.5: Antibiotic Classifications. Retrieved September, 8, 2024, from https://bio.libretexts.org/Courses/Northwest_University/MKBN211%3A_Introductory_Microbiology_(Bezuidenhout)/07%3A_Antimicrobial_Drugs/7.01%3A_Overview_of_Antimicrobial_Therapy/7.1.05%3A_Antibiotic_Classifications
CLSI. (2015a). Method for dilution antimicrobial susceptibility test for bacteria that grow aerobically: Approved standard. (10th edition). CLSI document M07-A10. Wayne, PA: Clinical laboratory standard institute.
CLSI. (2015b). Performance standards for antimicrobial disk susceptibility test; Approved standard (12th edition). CLSI document M02-A12. Wayne, PA: Clinical laboratory standard institute.
CLSI. (2023). Performance standards for antimicrobial disk susceptibility test; Approved standard. (33th edition). CLSI supplement M100. Wayne, PA: Clinical laboratory standard institute.
Gertsch, J. (2016). The Metabolic Plant Feedback Hypothesis: How Plant Secondary Metabolites Nonspecifically Impact Human Health. Planta Medica, 82(11-12), 920–929.
Gherardi, G. (2023). Staphylococcus aureus infection: pathogenesis and antimicrobial resistance. International Journal of Molecular Sciences, 24(9), 1-3.
Idris, F.Z., & Habibu, U.A. (2021). In-vitro antibacterial activity of Cinnamon bark extracts on clinical multi-drug resistant (MDR) Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa isolates. Bayero Journal of Pure and Applied Sciences, 14(1), 38-44.
Isah, T. (2019). Stress. & defense responses in plant secondary metabolites production. Biological Research, 52(39), 1-25.
Islam, M., Sabrin, M.S., Kabir, M.H.B., & Aftabuzzaman, M. (2018). Antibiotic sensitivity and resistant pattern of bacteria isolated from table eggs of commercial layers considering food safety issue. Asian Journal of Medical and Biological Research, 4(4), 323–329.
Mebkhout, F., Khelifi Touhami, N.A., Ouchene, N., Dahmane, T., Hamdi, T.M., & Kessi, O. (2022). Evaluation of bacterial contamination of laying hen eggshells by using a classic and fast method: First report in Algeria. Agricultural Science Technology, 14(4), 49–56.
Mogana, R., Adhikari, A., Tzar, M.N, Ramliza, R., & Wiart, C. (2020). Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates. BMC Complementary Medicine and Therapies, 20(1), 1-11.
Ngamsurach, P., & Praipipat, P. (2022). Comparative antibacterial activities of Garcinia cowa and Piper sarmentosum extracts against Staphylococcus aureus and Escherichia coli with studying on disk diffusion assay, material characterizations, and batch experiments. Heliyon, 8(11), 1-15.
Ngene, A.C., Aguiyi, J.C., Chibuike, C.J., Ifeanyi, V.O., Ukaegbu-Obi, K, M., Kim, E.G., Ohaeri, U.C., & Onyemegbulem, B.O. (2019). Antibacterial activity of Psidium guajava leaf extract against selected pathogenic bacteria. Advances in Microbiology, 9(12), 1012-1022.
Nimrat, S., Soodsawaeng, P., & Vuthiphandcha, V. (2018). Chemical Composition and Antibacterial Activity of Ethanol Extract of Lemon Grass (Cymbopogon citratus) on Pathogenic Bacteria: A review. Journal of Science and Technology Ubon Ratchathani University, 20(3), 20-28.
Oklo, A.D., Adah, C.A., Abah, C.N., Ode, P.I., Praise, A.J., & Ede, C.O. (2023). Physicochemical analysis and anti-bacterial activity of rhizome of tumeric (Curcuma longa L.) vegetable plants. World Journal of Advanced Research and Reviews, 2023, 18(01), 1169-1181.
Pondit, A., Haque, Z.F., Sabuj, A.A., Khan, M.R., & Saha, S. (2018). Characterization of Staphylococcus aureus isolated from chicken and quail eggshell. Journal of Advanced Veterinary and Animal Research, 5(4), 466-471.
Rikamalia, R., Rastina, R., & Ismail, I. (2018). Total Staphylococcus aureus in chicken eggs sold in minimarkets In Syiah Kuala district. Jurnal Ilmiah Mahasiswa Veteriner, 2(3), 388–395.
Syed, M.A., Shah, S.H.H., Sherafzal, Y., Shafi-Ur- Rehman, S., Khan, M.A., Barrett, J.B., Woodley, T.A., Jamil, B., Abbasi, S.A., & Jackson, C.R. (2018). Detection and molecular characterization of methicillin-resistant Staphylococcus aureus from table eggs in Haripur, Pakistan. Foodborne Pathogen and Disease, 15(2), 86–93.
Sithisarn, P., Rojsanga, P., & Sithisarn, P. (2021). Flavone-Rich Fractions and Extracts from Oroxylum indicum and Their Antibacterial Activities against Clinically Isolated Zoonotic Bacteria and Free Radical Scavenging Effects. Molecules, 26(1773), 1-20.
Thaha, A.H., Malaka, R., Hatta, W., & Maruddin, F. (2024). Staphylococcus aureus as a foodborne pathogen in eggs and egg products in Indonesia: A review. International Journal of One Health, 10(1), 141–147.
Tinrat, S. (2022). Phytochemical screenings, antibacterial and anti-biofilm activities of Garcinia cowa Roxb. leaves extracts and its synergistic effect with antibiotic. Pharmaceutical Sciences Asia, 49(6), 556-567.
Weldeselassie, M., Gugsa, G., Kumar, A., Tsegaye, Y., Awol, N., Ahmed, M., Abebe, M., & Tadele, H. (2020). Isolation and Characterization of Staphylococcus aureus From Food of Bovine Origin in Mekelle, Tigray, Ethiopia. The Open Microbiology Journal, 14, 234-241.
Yassin, M.T., Al-Askar, A.A., Mostafa, A. A. F., & El-Sheikh, M.A. (2020). Bioactivity of Syzygium aromaticum (L.) Merr. & LM Perry extracts as potential antimicrobial and anticancer agents. Journal of King Saud University-Science, 32(8), 3273-3278.
Yuceel-Timur, I., Garner, C.D., Franlin, S., & Hardy, D.J. (2024). Performance evaluation of cefoxitin screen test on two different automated antimicrobial susceptibility test systems: a comparative study. Microbiology Spectrum, 12(9), 1-6.
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