DEVELOPMENT OF BODY MASK PELLETS FROM MELALEUCA QUINQUENERVIA LEAVES EXTRACT CONTAINING ANTIBACTERIAL EFFECT

Abstract

Melaleuca quinquenervia (Cav.) S.T. Blake, commonly known as Cajuput, is a prominent species typically found in the southern region of Thailand. It has been found that Cajuput oil possesses antibacterial properties. Therefore, researchers were interested in developing a body mask pellet product from M.quinquenervia leaf extract. The study aimed to investigate the physical and chemical properties, short-term and long-term stability, antioxidant activity using the DPPH assay method, total phenolic compound content using the Folin-Ciocalteu method, antibacterial and antifungal using Broth dilution method, and cytotoxicity using the MTT assay. The body mask pellet formula consists of cassava starch, corn starch, polyvinylpyrrolidone-K30, croscarmellose sodium, preservatives, and M. quinquenervia leaf extract. The evaluation of short-term stability over 4 cycles revealed that the weight, pH, and odor changed slightly, while the color and texture remained unchanged. After 3 months, the pH value was 5.64±0.01, and the color and odor showed no alterations. The antioxidant activity of the body mask pellet product from M. quinquenervia leaf extract, compared with the standard ascorbic acid, showed an IC₅₀ values of 2.44 mg/mL and 2.36 µg/mL, respectively. The total phenolic compound content was 6.02±0.46 mg GAE/1 g Sample. The product exhibited inhibitory effects against bacteria and fungi (Staphylococcus epidermidis, Propionibacterium acnes, and Malassezia furfur) with the minimum inhibitory concentration (MIC) values of 32, 8, and 8 mg/mL, respectively. The minimum bactericidal concentration (MBC) values of 32, 32, and 32 mg/mL, respectively. When tested for cytotoxicity against Human Skin Fibroblast-hTERT cells, the test substance concentration was found to be nontoxic to cells <0.10 mg/mL. It can be further developed at the industrial level and lead to the creation of products that can add value to Thai herbs that can be found locally.