EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO CULTURE OF CHINESE GINGER (BOESENBERGIA ROTUNDA (L.) MANSF.)

Abstract

The objective was to study the effect of plant growth regulators on shoot multiplication and root induction of Chinese ginger at the Phichit Agricultural Research and Development Center. Sterilizing the young shoots of Chinese ginger and culturing them on MS medium without plant growth regulators for 4 weeks to initiate new shoots. After that, cultured shoot explants on MS medium supplemented with plant growth regulators. The experiment design was CRD with four treatments and four replications: 6-benzyladenine (BA), 6-furfurylaminopurine (Kinetin), 6-(γ,γ-dimethylallylamino) purine (2iP) or N-phenyl-N’-1,2,3-thidiazol-5-yl urea (TDZ) concentrations of 2 mg/L for 12 weeks. The result indicated that shoot explants cultured on MS medium supplemented with 2 mg/L TDZ for 4 weeks had shoot multiplication of 80.90%, a shoot number of 3.51 shoots, root induction of 26.30%, and a root number of 6.54 roots. After being cultured for 12 weeks on MS medium containing 2 mg/L TDZ, it has the highest shoot and root number at 7.48 shoots and 12.70 roots, respectively. Therefore, MS medium containing 2 mg/L TDZ is suitable for in vitro Chinese ginger to produce disease-free plants.