PROCESS FOR PREPARING HERBAL EXTRACTS FROM THONGNOPPAKUN WOUND HEALING FORMULA WITH ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES

Abstract

Thongnoppakun wound healing oil is a traditional medicinal formula used for wound healing. The extract from the Thongnoppakun wound healing formula possesses anti-inflammatory, antibacterial, antioxidant, and wound healing properties. This research aims to study the extraction methods of the Thongnoppakun wound healing formula. The antibacterial activity, antioxidant activity, and total phenolic content were studied. The stability of the Thongnoppakun wound healing extract was assessed. The results of the stability study under accelerated conditions. The experimental results showed that the physical characteristics of the extracts from all three extraction methods were highly similar. The extract obtained using microwave extraction at 300 W had a lighter yellow color. For the minimum inhibitory concentration; MIC and minimum bactericidal/fungicidal concentration (MBC/MFC) against Staphylococcus epidermidis, Propionibacterium acnes and Malassezia furfur, the extract obtained using microwave extraction at 800 W had MIC values of 4, 0.50, and 4 mg/mL, respectively. The MBC values were 4 and 0.50 mg/mL, while the MFC value was >32 mg/mL. Regarding antioxidant activity with DPPH method, the Thongnoppakun extract obtained using microwave extraction at 800 W had an IC₅₀ value of 0.37 mg/mL, compared to 1.59 µg/mL for standard ascorbic acid. Analysis of total phenolic content using Folin-Ciocalteu method revealed that the microwave 800 W extract had the highest phenolic content. For stability testing, all three extraction methods showed similar results, with precipitation occurring in the third cycle. These research findings can contribute to the development of Thongnoppakun extract-based wound healing products.