EFFICACY OF EMULSIFIED KAFFIR LIME PEEL ESSENTIAL OIL AGAINST SALMONELLA TYPHIMURIUM ON BASIL LEAVES
Keywords:
Kaffir lime peel essential oil, Emulsion, S. TyphimuriumAbstract
This study aimed to test the efficacy of emulsified kaffir lime peel essential oil against Salmonella Typhimurium (S. Typhimurium) on basil leaves. The minimum inhibitory concentration; MIC and minimum bactericidal concentration; MBC were determined by Macrobroth dilution. The MIC and MBC values were both 0.50%. The time-kill assay indicated that the emulsified kaffir lime peel essential oil concentration of 0.50% effectively killed >99.90% of the initial bacterial population within 5 minutes. Moreover, the antimicrobial activity of the emulsion against S. Typhimurium on basil leaves was tested. Compared to the control, the emulsion at concentrations of 0.50% (1xMIC) and 1% (2xMIC) showed significant reduction (P<0.05) in S.Typhimurium on the basil leaves. Wash treatment with 1%(2xMIC) exhibited greater significant effects on the pathogenic bacteria than 0.50% (1xMIC) (P<0.05). In addition, wash treatment with both concentrations of emulsified kaffir lime peel essential oil did not affect the color of basil leaves (P>0.05). The results from this study indicated that emulsified kaffir lime peel has efficacy in the decontamination of S. Typhimurium on basil leaves which can be applied to be as an alternative as a sanitizer for fresh vegetables.
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