THE FORMULATION FOR THROAT SPRAY FROM STINGLESS BEE HONEY PICKLED HERB FOR ANTIBACTERIAL ACTIVITY

Abstract

Stingless bee honey is an important substance consisting of phenolic and flavonoid groups that have the effect of inhibiting the growth of bacteria, anti-aging and wound healing in the respiratory system. According to traditional Thai medical wisdom, honey is commonly used in pickling herbs. In this research, stingless bee honey was developed to pickle herbs such as limes, lemongrass, and kaffir lime were used. The objectives of this research were to study the physical characteristics; and test the antibacterial activity of stingless bee honey pickled herbs and to develop a throat spray from stingless bee honey pickled herbs and accelerated stability test conditions. Results of a study on the physical characteristics of stingless bee honey pickled herbs, found that it is a yellowish-brown, non-separated liquid, sweet and sour taste, specific fragrance, and pH=6.65. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) values of S. aureus, S. pyrogens and S. pneumonia were 1.56-3.13 %v/v. The F4 was the best formula which consisted of stingless honey pickled herbs 4.40 mL, 95% ethyl alcohol 0.40 mL, menthol 0.08 g, sodium benzoate 0.004 g, poloxamer 1 g and boiled water qs to 20 mL. For the results of the accelerated stability test, it was found that the pH and precipitation were not significantly slightly changed. There were no changes in color, and taste and foreign matter to be found. Therefore, throat sprays from stingless bee honey pickled herb will be further developed so that the formula and manufacturing processes can be transferred to entrepreneurs in the future.