ANTIOXIDANT ACTIVITY TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS OF MELIENTHA SUAVIS FRUIT

Abstract

This research is aimed to study the antioxidant activity of Melientha suavis fruit pulp extract. The dried fruit pulp was macerated with methanol. After solvent evaporation, the extract was tested for antioxidant activity by using DPPH and ABTS methods which determined using vitamin C and Trolox as standards. The result showed that the extract exhibited antioxidant activity against DPPH and ABTS with IC₅₀ values of 32.78 and 30.06 mg/mL, respectively, which calculated as Vitamin C Equivalent Antioxidant Capacity (VCEAC) values were 1.52 and 1.00 mg vitamin C/g extract, respectively. The antioxidant activity showed Trolox Equivalent Antioxidant Capacity (TEAC) values of 1.83 and 0.66 mg Trolox/g extract, respectively. Total phenolic and total flavonoid contents were 209.0±44.6 mg GAE/g extract and 109.5±49.5 mg QE/g extract, respectively. The fruit extract was found to contain antioxidant compounds phenolics and flavonoids with a high antioxidant activity value. Therefore, it should be developed and used as food products, beverages such as tea powder, or applied as an ingredient in other foods.