DETECTION OF COLLETOTRICHUM GLOEOSPORIOIDES IN “NAM DOK MAI” MANGO USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METHOD

Authors

  • Dueantem Thongphueak Rambhai Barni Rajabhat University
  • Sasithorn Bhudharak
  • Jirapat Chanthamalee

Keywords:

Anthracnose, DNA biosensor, LAMP

Abstract

The molecular genetic techniques for detection of Colletotrichum gloeosporioides in “Nam Dok Mai” mango was studied. The objective of this research was to develop analytical methods for C. Gloeosporioides of causing anthracnose in Nam Dok Mai mango by using Loop-Mediated Isothermal Amplification (LAMP) technique. In this research, the characteristics of C. gloeosporioides was analyzed by LAMP method. The sensitivity and specificity of C. Gloeosporioides were also investigated. The study of the anthracnose disease symptoms in “Nam Dok Mai” mango which caused by Colletotrichum gloeosporioides infection showed that slow colony growth on potato dextrose agar; PDA grayish white colony, Single cell conidia, oval and colorless and cylindrical spores. The determination of LAMP, It was found that the analyzed from the experiments 20 samples. The study of the sensitivity of DNA detection was found at 50x10-4 µg/ml. The detection of specificity for other bacteria was found that it did not show any cross-reactivity with other bacterial species. The LAMP technique is a highly accurate DNA at the molecular biology and rapid test. The analyzed most number of samples by using a few of DNA.

References

Arauz, L.F. (2000). Mango Anthracnose: Economic Impact and Current Options For Integrated Managaement. Plant Dis, 84(6), 600-611.

De Guzman Alvindia, D., & Mangoba, M.A.A. (2020). Bioactivities of Allium longicuspis Regel against anthracnose of mango caused by Colletotrichum gloeosporioides (Penz.). Sci Rep, 10(1), 11367.

Fu, F.F., Hao, Z., Wang, P., Lu, Y., Xue, L. J., Wei, G., . . . Chen, J. (2020). Genome Sequence and Comparative Analysis of Colletotrichum gloeosporioides Isolated from Liriodendron Leaves. Phytopathology, 110(7), 1260-1269.

Fu, S., Qu, G., Guo, S., Ma, L., Zhang, N., Zhang, S., . . . Shen, Z. (2011). Applications of loop-mediated isothermal DNA amplification. Appl Biochem Biotechnol, 163(7), 845-850.

Ganogpichayagrai, A., Rungsihirunrat, K., Palanuvej, C., & Ruangrungsi, N. (2016). Characterization of Mangifera indica cultivars in Thailand based on macroscopic, microscopic, and genetic characters. J Adv Pharm Technol Res, 7(4), 127-133.

Harju, S., Fedosyuk, H., & Peterson, K.R. (2004). Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC Biotechnol, 4, 8.

Kwodaga, J.K., Sowley, E.N.K., & Badii, B.K. (2020). Morphological and molecular characterisation of Colletotrichum gloeosporioides (Penz) isolates obtained from Dioscorea rotundata (Poir). African Journal of Biotechnology, 19(5), 231-239.

Lan, C., Yao, J., Yang, X., Ruan, H., Yu, D., & Jiang, J. (2020). Specific and sensitive detection of the guava fruit anthracnose pathogen (Colletotrichum gloeosporioides) by loop-mediated isothermal amplification (LAMP) assay. Can J Microbiol, 66(1), 17-24.

Mori, Y., Nagamine, K., Tomita, N., & Notomi, T. (2001). Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun, 289(1), 150-154.

Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Res, 28(12), E63.

Ren, Y., Xue, Y., Tian, D., Zhang, L., Xiao, G., & He, J. (2020). Improvement of Postharvest Anthracnose Resistance in Mango Fruit by Nitric Oxide and the Possible Mechanisms Involved. J Agric Food Chem, 68(52), 15460-15467.

Tovar-Pedraza, J.M., Mora-Aguilera, J.A., Nava-Diaz, C., Lima, N.B., Michereff, S.J., Sandoval-Islas, J.S., . . . Leyva-Mir, S.G. (2020). Distribution and Pathogenicity of Colletotrichum Species Associated With Mango Anthracnose in Mexico. Plant Dis, 104(1), 137-146.

Wang, S., Ye, W., Tian, Q., Dong, S., & Zheng, X. (2017). Rapid detection of Colletotrichum gloeosporioides using a loop-mediated isothermal amplification assay. Australasian Plant Pathol, 46, 493–498.

Wu, J.Y., Hu, X.R., & Zhang, C.Q. (2019). Molecular Detection of QoI Resistance in Colletotrichum gloeosporioides Causing Strawberry Anthracnose Based on Loop-Mediated Isothermal Amplification Assay. Plant Dis, 103(6), 1319-1325.

Downloads

Published

2021-12-13

How to Cite

Thongphueak, D., Bhudharak, S. . ., & Chanthamalee, J. . . (2021). DETECTION OF COLLETOTRICHUM GLOEOSPORIOIDES IN “NAM DOK MAI” MANGO USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) METHOD. PSRU Journal of Science and Technology, 6(3), 94–105. Retrieved from https://ph01.tci-thaijo.org/index.php/Scipsru/article/view/245301

Issue

Vol. 6 No. 3 (2021): September - December 2021

Section

Research Articles

License

กองบรรณาธิการขอสงวนสิทธิ์ในการปรับปรุงแก้ไขตัวอักษรและคำสะกดต่างๆ ที่ไม่ถูกต้อง และต้นฉบับที่ได้รับการตีพิมพ์ในวารสาร PSRU Journal of Science and Technology ถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม และ
ผลการพิจารณาคัดเลือกบทความตีพิมพ์ในวารสารให้ถือมติของกองบรรณาธิการเป็นที่สิ้นสุด