DETECTION OF CAMPYLOBACTER JEJUNI USING COLORIMETRIC OF LOOP-MEDIATED ISOTHERMAL AMPIFICATION FOR CHICKEN MEAT IN MUANG DISTRICT CHANTHABURI PROVINCE
Keywords:
Campylobacter spp., Loop-mediated isothermal amplification, ColorimetricAbstract
The objectives of this research were detecting the Campylobacter jejuni using colorimetric of loop-mediated Isothermal amplification (LAMP) compared with the standard method. Thirty chicken samples were collected from markets in Muang district, Chanthaburi province, Thailand. The results showed 13 samples of chicken meat were contaminated with C. jejuni. However, 17 samples were not found the contamination of C. jejuni and the lowest sensitivity of DNA detection was 364 fg µl-1. The detection of specificity for other bacteria was found that the primers did not show any cross-reactivity with other pathogenic bacteria. The standard method was compared with the LAMP DNA method, it was shown that both methods had the sensitivity, specificity and 100 percent accuracy. The detection kit has been developed for easy to use, comfortable, rapid and accuracy in the diagnosis of C. jejuni. Moreover, this test kit can be used for detection in the field for surveillance of Campylobactor spp. in poultry products.
References
Frirdich, E., Biboy, J., Huynh, S., Parker, C.T., Vollmer, W., & Gaynor, E.C. (2017). Morphology heterogeneity within a Campylobacter jejuni helical population: the use of calcofluor white to generate rod-shaped C. jejuni 81-176 clones and the genetic determinants responsible for differences in morphology within 11168 strains. Mol Microbiol, 104(6), 948-971.
Hajihosseini, S., Nasirizadeh, N., Hejazi, M.S., & Yaghmaei, P. (2016). A sensitive DNA biosensor fabricated from gold nanoparticles and graphene oxide on a glassy carbon electrode. Mater Sci Eng C Mater Biol Appl, 61, 506-515.
Hiroshi Ushijima, S.N., Thongprachum, A., Shimizu-Onda, Y., Tran, D.N., Pham, N.T.K., Takanashi, S., Dey, S.K., Okitsu S., Yamazaki, W., Mizuguchi, M., & Hayakawa, S. (2014). Sensitive and rapid detection of campylobacter species from stools of children with diarrhea in Japan by the loop-mediated isothermal amplification method. Japanese Journal of Infectious Diseases, 67(5), 374–378.
Kaakoush, N.O., Castano-Rodriguez, N., Mitchell, H.M., & Man, S.M. (2015). Global Epidemiology of Campylobacter Infection. Clin Microbiol Rev, 28(3), 687-720.
Kaygusuz, D., Vural, S., Aytekin, A.O., Lucas, S.J., & Elitas, M. (2019). DaimonDNA: A portable, low-cost loop-mediated isothermal amplification platform for naked-eye detection of genetically modified organisms in resource-limited settings. Biosens Bioelectron, 141, 111409.
Lawson, A.J., Logan, J.M., O'Neill G.L., Desai, M., & Stanley, J. (1999). Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay. J Clin Microbiol, 37(12), 3860-3864.
Mohran, Z.S., Arthur, R.R., Oyofo, B.A., Peruski, L.F., Wasfy, M.O., Ismail, T.F., & Murphy, J.R. (1998). Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA. Appl Environ Microbiol, 64(1), 363-365.
Pham, N.T., Trinh, Q.D., Khamrin, P., Ukarapol, N., Kongsricharoern, T., Yamazaki, W., Komine-Aizawa, S., Okitsu, S., Maneekarn, N., Hayakawa, S., & Ushijima, H. (2015). Loop-Mediated Isothermal Amplification (LAMP) for Detection of Campylobacter jejuni and C. coli in Thai Children with Diarrhea. Jpn J Infect Dis, 68(5), 432-433.
Quyen, T.L., Nordentoft, S., Vinayaka, A.C., Ngo, T.A., Engelsmenn, P., Sun, Y., Madsen, M., Bang, D.D., & Wolff, A. (2019). A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection of Campylobacter spp. in Broiler Production. Front Microbiol, 10, 2443.
Romero, M.R., D'Agostino, M., Arias, A.P., Robles, S., Casado, C.F., Iturbe, L.O., Lerma, O.G., Andreou, M., & Cook, N. (2016). An immunomagnetic separation/loop-mediated isothermal amplification method for rapid direct detection of thermotolerant Campylobacter spp. during poultry production. J Appl Microbiol, 120(2), 469-477.
Sabike, II., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto Y., & Yamazaki, W. (2016). Use of Direct LAMP Screening of Broiler Fecal Samples for Campylobacter jejuni and Campylobacter coli in the Positive Flock Identification Strategy. Front Microbiol, 7, 1582.
Silva, J., Leite, D., Fernandes, M., Mena, C., Gibbs, P.A., & Teixeira, P. (2011). Campylobacter spp. as a Foodborne Pathogen: A Review. Front Microbiol, 2, 200.
Szczepanska, B., Andrzejewska, M., Spica, D., & Klawe, J.J. (2017). Prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from children and environmental sources in urban and suburban areas. BMC Microbiol, 17(1), 80.
Xie, L., Xie, Z., Zhao, G., Liu, J., Pang, Y., Deng, X., Xie, Z., Fan, Q., & Luo, S. (2014). A loop-mediated isothermal amplification assay for the visual detection of duck circovirus. Virol J, 11, 76.
Yamazaki, W. (2013). Sensitive and rapid detection of Campylobacter jejuni and Campylobacter coli using loop-mediated isothermal amplification. Methods Mol Biol, 943, 267-277.
Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K., & Misawa, N. (2009). Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples. Appl Environ Microbiol, 75(6), 1597-1603.
Downloads
Published
How to Cite
Issue
Section
License
กองบรรณาธิการขอสงวนสิทธิ์ในการปรับปรุงแก้ไขตัวอักษรและคำสะกดต่างๆ ที่ไม่ถูกต้อง และต้นฉบับที่ได้รับการตีพิมพ์ในวารสาร PSRU Journal of Science and Technology ถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม และ
ผลการพิจารณาคัดเลือกบทความตีพิมพ์ในวารสารให้ถือมติของกองบรรณาธิการเป็นที่สิ้นสุด
0-5526-7000 Ext. 4100
