Thunbergia laurifolia and Phyllanthus amarus Schum & Thonn. Extract decrease interleukin-1 levels secretion in LPS-Activated THP-1 Macrophages

Authors

  • atittaya rocejanasaroj

Keywords:

Thunbergia laurifolia, Phyllanthus amarus Schum & Thonn, Interleukin-1, Macrophage, Chronic inflammation

Abstract

Inflammation is the complex immune response evoked by infections or tissue injury. When inappropriate or uncontrollable inflammation occurs, it acts as a strong disease-promoting factor in a number of pathological degenerative disorders. Nonsteroidal anti-inflammatory drugs (NSAIDs) have been currently used to treat inflammatory based disorders but may cause complications and undesired adverse effects mainly on gastrointestinal system and cardiovascular system. As the result, increasing studies focusing on plant-derived natural products and their derivatives, many new plant-derived extracts and active compounds have been identified to exhibit anti-inflammatory effects. The aim of this study was to examine the effect of Thunbergia laurifolia extracts (TLE) and Phyllanthus amarus extracts (PAE) on pro-inflammatory cytokine IL-1 secretion in human macrophage-like THP-1 cells. Cells were treated with TLE 200-800 μg/ml, PAE 200-800 μg/ml, 2 well known anti-inflammatory agents : Epigallocatechin gallate (EGCG) (50 nM) and IL-10 (10 ng/ml) with LPS (1,000 ng/ml) used as inflammatory mediator for 24 hrs. Exposure cells with 1,000 ng/ml LPS was significantly induced IL-1 secretion (p=0.000). Co-treatment LPS with herbal extracts revealed that TLE 400-800 μg/ml and PAE 200-800 g/ml significantly reduced IL-1 secretion in LPS-treated cells (p=0.000) in dose dependent fashion. However, we didn’t find significant inhibitory effect of EGCG (50 nM) and IL-10 (10 ng/ml) on LPS induced IL-1 secretion. As such, TLE and PAE could be the potential therapeutic natural products for the treatment of inflammasome-dependent diseases and may serve as lead herb for the development of new anti-inflammatory drugs.

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Published

2020-06-29