Quantitative thin layer chromatographic analysis of rosmarinic acid as a chemical marker in Perilla frutescens leaves Parichart Hongsing, Chanida Palanuvej* and Nijsiri Ruangrungsi
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Abstract
The simple, less time consuming and selective methods for determination of rosmarinic acid content in Perilla frutescens (L.) Britton leaf were developed using TLC-densitometry and TLC-image analysis. Additionally, rosmarinic acid as a chemical marker in Perilla frutescens leaf extracts could be used to strengthen the quality of the starting herbal raw materials of Perilla frutescens leaf. Perilla frutescens leaves were collected from 15 different locations in Thailand. The analytical separation was performed on the silica gel60 GF254 TLC plate. Two mobile phases were optimized for this study. The first one was toluene-chloroform-acetone-formic acid (5:4:1:0.2) and the second one was toluene-ethyl acetate-formic acid (5:4:1). Both quantitative TLC methods to determine rosmarinic acid content in the leaf provided valid specificity, accuracy, repeatability, intermediate precision, limit of detection, limit of quantitation and robustness. Quantitative TLC of rosmarinic acid revealed 2.504 ± 1.631 and 2.485 ± 1.606 g per 100 g of dried leaves by densitometry and image analysis respectively. TLC-image analysis could be one of alternative methods of rosmarinic acid quantification in Perilla frutescens leaf.
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ลิขสิทธิ์ต้นฉบับที่ได้รับการตีพิมพ์ในวารสารนวัตกรรมวิทยาศาสตร์เพื่อการพัฒนาอย่างยั่งยืนถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยสวนดุสิต ห้ามผู้ใดนำข้อความทั้งหมดหรือบางส่วนไปพิมพ์ซ้ำ เว้นแต่จะได้รับอนุญาตอย่างเป็นลายลักษณ์อักษรจากคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยสวนดุสิต นอกจากนี้ เนื้อหาที่ปรากฎในบทความเป็นความรับผิดชอบของผู้เขียน ทั้งนี้ไม่รวมความผิดพลาดอันเกิดจากเทคนิคการพิมพ์
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