Chemical Composition and Toxicity of Peel of Native Species Mango in Sa Kaeo Province Against Spodoptera litura
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Abstract
The purpose of this research was to study the phytochemical screening, antioxidation and efficiency to control Spodoptera litura of mango peel which is a native species in Sa Kaeo province, Thailand. According to phytochemical preliminary study of mango peel, 5 types were found: alkaloids, flavonoids, coumarins, tannins and terpenoids. The extract of mango peel with ethyl acetate solvent showed the highest total phenolic content and total tannins content which were 403.10±0.02 mg GAE/g and 330.78 ±0.02 mg TAE/g, respectively. For total flavonoids content, the highest was found in dichloromethane extract at 255.11±0.02 mg QE/g. The antioxidant activity study obtained from DPPH free radical scavenging showed the highest in methanol extract (EC50 value was 4.38±0.03 μg/ml). In the addition, it was found that the contact toxicity test by topical application of mango peel extract was able to control Spodoptera litura. In which the active substance extracted with ethyl acetate, it showed the best effect at 24 and 48 hours (2,392.14±0.13 and 1,580.26±0.12 mg/l, respectively). Therefore, ethyl acetate extract was analyzed by high performance liquid chromatography (HPLC), in which only gallic acid was found. It can control many bacteria and insects. Overall results suggest that mango peels from Sa Kaeo province can be further studied for using in insect control plans to reduce the use of chemical pesticides.
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