A Cost-Effective and Environmentally Friendly Approach in Using Green Tea Extracts for The Determination of Iron Ion with PiCOEXPLORER
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Abstract
This research has been developed by using green tea extracts as a green reagent for the determination of iron ion in pharmaceutical products with PiCOEXPLORER as a detector. PiCOEXPLORER is a modern instrument based on the colorimetric system. It is a compact system and a portable device of a new generation. It is a small size colorimeter based on the RGB analysis system which allows displaying RGB intensity and absorbance for each Red (R), Green (G), and Blue (B) mode. The device is controlled by a program using an application on a smartphone with either iOS or Android operating systems. This portable device is convenient for on-site micro-scale analysis. Green tea extracts can be used as an alternative low cost and easily available reagent for the quantification of iron without purifying extraction before use. Parameters affecting the determination of iron content were optimized. Under the optimum conditions developed herein, the results showed good linearity over a Fe(III) concentration range from 2.5 to 30.0 mg×L–1 gave correlation coefficients of 0.9985 (y = 0.0093x–0.002) with a detection limit (LOD) of 0.5 mg×L–1 and quantification limit (LOQ) of 1.3 mg×L–1. The repeatability of the proposed method was less than 4 %RSD (n = 7). The proposed method was successfully applied to pharmaceutical products. The results matched well with those obtained from the standard method as compared at a 95% confidence level with the paired t-test. The proposed method is common, cost-effective, reduces the sample and reagent consumption, and is an environmentally friendly method. In addition, this device could be used as an alternative tool for spectroscopy study in terms of quantitative analysis instead of using a conventional spectrophotometer.
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