Crude Cllulase Powder Production by Solid-State Fermentation Using Cassava Residue and Co-cultured Microorganisms Trichoderma reesei and Saccharomyces cerevisiae

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Pongsri Siwarasak
Sathaporn Thongwik


This research deals with the production of fresh crude cellulase by solid-state fermentation (SSF) using cassava residue and a co-culture of Trichoderma reesei and Saccharomyces cerevisiae under cassava residue weight, liquid medium volume. The fresh product from SSF was performed for moisture removal until crude cellulase obtained after grinding.  Submerged fermentation was investigated using lignocellulosic materials as a substrate suspended in cultivation medium and then mixing with cellulase powder. In the production of fresh crude cellulase, the experiment began of 100 g cassava residuemoisture content 12% w/w and the co-cultured microorganisms in pH 5 liquid medium (LM) of 107 cell/mL. To find the suitable moisture content for SSF, 100 g cassava residuemoisture content 12% w/w to LM was tested at ratios of 1:0.6, 1:0.8 and 1:1 w/v, initial sugar concentration in LM was varied between 0.8, 1.6, 2.4 and 3.2%w/w and initial cell concentration is constant. The solid-state fermentation was carried out at  C for 7 days. The optimal condition of SSF are 3.2% w/w initial sugar concentration in 100 mL LM100g cassava residue and 6 days of incubation at C. The fresh crude cellulase with 55% w/w moisture content was subsequently dehydrated and pulverized to produce crude cellulase powder with 12% w/w. The powder was applied to dried pineapple peels fermentation for ethanol production.


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