APPLICATION OF PECTINASE AND CELLULASE IN SYRUP DEVELOPMENT FROM DRIED HOM THONG BANANA

Authors

  • Ataya Santakul Faculty of Science and Technology, Sathon District, Rajamangala University of Technology Bangkok
  • Savarak Chantaratheptimakul Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand
  • Direk Boonthum Division of Physics, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand
  • Naruemon Jommark Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand
  • Suwit Kongsakorn Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep

DOI:

https://doi.org/10.14456/lsej.2026.10

Keywords:

Dried Hom Thong, Pectinase, Cellulase, Syrup

Abstract

This study aimed to determine the optimal conditions for syrup production from dried Hom Thong banana using pectinase and cellulase, as well as to evaluate the physicochemical properties and sensory acceptability of the developed product. Dried Hom Thong banana was extracted using pectinase and cellulase at concentrations ranging from 0.05–0.15% (w/w). The results indicated that the optimal condition was the combination of 0.10% pectinase and 0.15% cellulase, which yielded the highest extraction yield, total soluble solids (TSS), and clarity (% transmittance at 670 nm; %T670). The extracted juice was subsequently concentrated under vacuum at 60 and 70°C to produce syrup. The resulting syrup had TSS values in the range of 72–74 °Brix, complying with the standard for fruit syrups. In terms of physicochemical properties, lightness (L*) decreased, while redness (a*) and yellowness (b*) increased with increasing evaporation temperature. Reducing sugar content significantly increased (p < 0.05), whereas total phenolic content decreased. Sensory evaluation revealed that the syrup produced under the optimal condition received high acceptance scores, particularly in color, clarity, and overall acceptability. These findings demonstrate that the combined use of pectinase and cellulase, together with vacuum concentration, can effectively enhance extraction efficiency and improve product quality. This approach also shows strong potential for commercial application, adding value to dried Hom Thong banana.

 

Author Biographies

Savarak Chantaratheptimakul, Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand

Lecturer, Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep.

Direk Boonthum, Division of Physics, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand

Lecturer, Division of Physics, Faculty of Science and Technology, Rajamangala University of Technology Krungthep.

Naruemon Jommark, Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand

Lecturer, Division of Food Safety Management and Technology, Faculty of Science and Technology, Rajamangala University of Technology Krungthep.

References

Abdella MA, Ibrahim GE. Application of statistical designs strategy to improve cellulase production using agro-waste residue by a novel isolate Bacillus licheniformis strain-MA1 and assessing the enzyme effect on apple juice quality. BMC microbiology 2024;24(1):511.

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols 2007;2(4):875-877.

Anese M, Nicoli MC, Massini R, Lerici CR. Effects of drying processing on the Maillard reaction in pasta. Food Research International 1999;32(3):193-199.

AOAC. Official methods of analysis of the Association of Official Analytical Chemists. City: AOAC International; 2000.

Argo BD, Damayanti C, Wulandari A, Mianadhiroh U. Effect of temperature and duration of thermal pasteurization on polyphenol oxidase (PPO) enzyme activity, total plate count (TPC), physicochemical and organoleptic properties of Cavendish banana fruit juice (Musa cavendishii). Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) 2021;4(2):117-130.

Arsad P, Sukor R, Wan Ibadullah W, Mustapha N, Meor Hussin A. Effects of enzymatic treatment on physicochemical properties of sugar palm fruit juice. International Journal on Advanced Science, Engineering and Information Technology 2015;5(5):308-312.

Bhardwaj N, Kumar B, Agrawal K, Verma P. Current perspective on production and applications of microbial cellulases: a review. Bioresources and Bioprocessing 2021;8(1):95.

Chantaratheptimakul S, Santakul A, Boontham D, Jommark N. Effect of egg albumin concentration and carboxymethyl cellulose on properties of hom-thong banana [Musa acuminata (AAA group)'Gros Michel'] foam snack produced by foam mat drying. International Food Research Journal 2025;32(2).

Chavan U, Prabhukhanolkar A, Pawar V. Preparation of osmotic dehydrated ripe banana slices. Journal of food science and technology 2010;47(4):380-386.

Cho J-S, Lee H-J, Park J-H, Sung J-H, Choi J-Y, Moon K-D. Image analysis to evaluate the browning degree of banana (Musa spp.) peel. Food chemistry 2016;194:1028-1033.

Codex Alimentarius Commission. Codex alimentarius. City: Food & Agriculture Org.; 1992.

da Mota RV, Lajolo FM, Cordenunsi BR, Ciacco C. Composition and functional properties of banana flour from different varieties. Starch‐Stärke 2000;52(2‐3):63-68.

De Lange R. Enzymatic extraction and clarification of banana juice (Musa sapientum Linn. var. suaveolus); 1988.

Dowerah SS, Das A, Singla M, Sit N. Comparison of yield and properties of banana juice extracted by hot water extraction method and different enzymes and preparation of wine from the extracted juices. Food Bioengineering 2023;2(4):317-327.

Fernando H, Srilaong V, Pongprasert N, Boonyaritthongchai P, Jitareerat P. Changes in antioxidant properties and chemical composition during ripening in banana variety'Hom Thong'(AAA group) and'Khai'(AA group). International Food Research Journal 2014;21(2):749.

Hafizov SG, Hafizov GK. Effect of heat treatment and pH factor on phenolic components of pomegranate juice. Journal of Hygienic Engineering & Design 2020;33.

Handique J, Bora SJ, Sit N. Optimization of banana juice extraction using combination of enzymes. Journal of food science and technology 2019;56(8):3732-3743.

Ismail BP. Nielsen's Food Analysis Laboratory Manual. City: Springer; 2024.

Koffi E, Sims C, Bates R. Viscosity reduction and prevention of browning in the preparation of clarified banana juice. Journal of Food Quality 1991;14(3):209-218.

Kumar R, Singh AK, Yadav SK, Kumar M. Response Surface Optimization Of Banana (Musa Sp.) Fruit Juice Clarification Using Multi-Enzyme System (Pectinase, Cellulase And Hemicellulase). Journal of Namibian Studies 2023;35.

Mohapatra D, Mishra S, Singh CB, Jayas DS. Post-harvest processing of banana: opportunities and challenges. Food and bioprocess technology 2011;4(3):327-339.

Molaee Parvarei M, Khorshidian N, Yousefi M, Zendeboodi F, Mirsaeedghazi H. Effect of membrane clarification on the physicochemical properties of fruit juices: A review. Iranian Journal of Chemistry and Chemical Engineering 2021.

Pongprasert N, Srilaong V. A novel technique using 1-MCP microbubbles for delaying postharvest ripening of banana fruit. Postharvest Biology and Technology 2014;95:42-45.

Qamar S, Shaikh A. Therapeutic potentials and compositional changes of valuable compounds from banana-A review. Trends in Food Science & Technology 2018;79:1-9.

Rasoulian SS, Aminifar M, Rashidi L. Investigating the effect of two different thermal methods of rotary evaporation and microwave on the phenolic content, antioxidant activity and color of grapefruit juice concentrate. 2017.

Ribeiro DS, Henrique SM, Oliveira LS, Macedo GA, Fleuri LF. Enzymes in juice processing: a review. International Journal of Food Science and Technology 2010;45(4):635-641.

Sharma HP, Patel H, Sharma S. Enzymatic extraction and clarification of juice from various fruits–a review. Trends Post Harvest Technol 2014;2(1):1-14.

Tadakittisarn S, Haruthaithanasan V, Chompreeda P, Suwonsichon T. Optimization of pectinase enzyme liquefaction of banana ‘Gros Michel’for banana syrup production. Agriculture and Natural Resources 2007;41(4):740-750.

Tapre A, Jain R. Effect of enzymatic treatment on juice yield, viscosity and clarity of banana juice. Asian Journal of Dairy and Food Research 2014;33(1):25-31.

Downloads

Published

2026-05-21

How to Cite

Santakul, A., Chantaratheptimakul, S., Boonthum, D., Jommark, N., & Kongsakorn, S. (2026). APPLICATION OF PECTINASE AND CELLULASE IN SYRUP DEVELOPMENT FROM DRIED HOM THONG BANANA. Life Sciences and Environment Journal, 27(1), 141–157. https://doi.org/10.14456/lsej.2026.10

Issue

Section

Research Articles