CHARACTERIZATION OF MANNANASE FROM POTENTIAL MANNAN-DEGRADING BACTERIA
DOI:
https://doi.org/10.14456/lsej.2025.4Keywords:
enzyme characterization, mannan, mannanase, mannanase-producing bacteriaAbstract
Mannanase is an enzyme that catalyzes the hydrolysis of mannan polysaccharides and has applications in diverse industries. It can be produced by various microorganisms, particularly bacteria, with each strain exhibiting unique enzymatic properties. This study aimed to select a potential mannanase-producing bacterial strain from isolates obtained from coconut residue-rich sites. In addition, identification of bacterial strain and enzyme characterization were performed. The results showed that isolate KD109 exhibited the highest enzyme activity at 1.58 U/ml. Based on 16S rRNA gene sequencing, KD109 showed over 99% sequence identity with Klebsiella pneumoniae. Characterization of the mannanase produced by this strain revealed that the optimum temperature of 50 °C and the optimum pH of 6. Substrate specificity analysis showed that the enzyme exhibited the highest specificity for mannan-based substrates, including konjac, followed by locust bean gum. Furthermore, this enzyme exhibited the activity on copra meal, a mannan rich by-product. This study indicated that K. pneumoniae KD109 could be a promising source of mannanase with potential applications in the degradation of mannan-containing raw materials for various industrial processes.
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