ANTIBACTERIAL PROPERTIES BIOFILM FORMATION INHIBITION AND CHEMICAL PROFILING OF AMPELOCISSUS ARACHNOIDEA
DOI:
https://doi.org/10.14456/lsej.2025.6Keywords:
antibacterial, biofilm, wild grape, crude extract, phytosterolsAbstract
This research aimed to analyze the chemical composition and evaluate the biological activities of wild grape leaves and stems extracted with ethanol. Chemical composition was determined using Gas Chromatography-Mass Spectrometry (GC-MS), revealing that the primary compounds in leaves and stems were plant sterols, notably g-Sitosterol, and other related compounds. Furthermore, the extracts were tested against five pathogenic bacterial strains: Escherichia coli SK005, Pseudomonas aeruginosa SK002, Klebsiella pneumoniae SK001, Staphylococcus aureus SK004, and Streptococcus pyogenes SPV01, using the disc diffusion assay. The results showed that the wild grape extracts inhibited the growth of all tested bacterial strains, with inhibition zone diameters ranging from 7.40±0.11 to 18.94±0.35 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined between 3.13 and 50.00 mg/mL. Additionally, the extracts exhibited biofilm inhibition activity against S. pyogenes, reaching up to 79.54%.
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