• Wanpen Khongpet Faculty of Science and Technology, Nakhon Sawan Rajabhat University
  • Phontip Sanghkawisu Faculty of Science and Technology, Nakhon Sawan Rajabhat University
  • Watcharasak Makerd Faculty of Education, Nakhon Sawan Rajabhat University
  • Maneerat Namjan Faculty of Science and Technology, Nakhon Sawan Rajabhat University



Curcuma putii Maknoi & Jenjitt, Phytochemical screening, Total phenolic content, Antioxidant activity, Tannin content


Curcuma putii Maknoi & Jenjitt. is a local medicinal plant, found only in Nongbua district, Nakhon Sawan province. This research aimed to study phytochemical screening, total phenolic content, and antioxidant activity of the C. putii Maknoi & Jenjitt. extracts. The phytochemical screening was investigated using the standard method based on color change reaction and precipitation. The total phenolic content was determined using The Folin-Ciocalteu method. The antioxidant activity was tested with the DPPH Radical Scavenging method. The results revealed that
C. putii Maknoi & Jenjitt. contained a wide variety of phytochemicals in its various parts. Tannin was found in leaves and had a total tannin content of 325.5 mg/L. The C. putii Maknoi & Jenjitt. extracts showed the highest total phenolic content in leaves (340.89 mg/L). They exhibited inhibition of antioxidants, with a 50% inhibition (IC50) at a concentration of 54.12 mg/mL, compared to the extracts from other parts of C. putii Maknoi & Jenjitt. It could be concluded that leaf extract is highly effective as an antioxidant. Therefore, the proposed plant is an interesting approach for developing healthcare products in future studies.


AOAC. Official methods of analysis. Association of official analytical chemists; 2000.

Buachoon N, Antioxidant activity and total phenolic content from crude extracts of morinda citrifoloa. VRU Research and Development Journal Science and Technology 2020;15(2):67-75.

Halee A, Rattanapun B. Study of antioxidant efficacies of 15 local herbs. Kmutt Research and Development Journal 2020;40(2):283-293.

Leite L, Dourado L. Laboratory activities, science education and problem-solving skills. Procedia- Social and Behavioral Sciences. 2013;106:1677-1686.

Maknoi C, Ruchisansakun S, Jenjittikul T. Curcuma putii (Zingiberaceae), A New Species from Thailand. Annales Botanici Fennici 2019;56(4-6):351-353.

Naiumsawang W, Phytochemicals and biological activities of medicinal plants. The Science Journal of Phetchaburi Rajabhat University 2018;15(2):51-60.

Nupan B, Thipwong J, Saisa-ard K, Samala S. Phytochemical and antibacterial studies of the crude leaves’ extract from Limnophila rugosa (Roth) Merr. Songklanakarin Journal of Plant Science 2016;3(3):26-33.

Piwpan P, Screening and isolation of microorganisms to improve the nutritional value of fermented soybean meal for animal feed. Master of Science (Biotechnology)/ Department of Biotechnology Graduate School, Silpakorn University; 2017.

Preeyachit S. An Optimal condition of oil extraction from jatropha curcas seed kernels using solvent extraction. Master of Science (Environmental Management), School of Social and Environmental Development, National Institute of Development Administration; 2008.

Rak-archa S, Pongamornkul W, Somnoo T, Tanming W, Wongkhak M. Ginger’s family in queen sirikit botanic garden. Chiang Mai: The Botanical Garden Organization (Ministry of Natural Resources and Environment); 2020.

Ruenwai R, Analysis of phenolic content and free radical scavenging capacity of Chrysanthemum flower extracts. Naresuan Phayao Journal 2020;13(1):16-20.

Seekhaw P, Chuaboonmee R, Surayot P, Chadpan S, Thurnkul N. Evaluation of phytochemical screening, antioxidant and antimicrobial activities from ethanolic extracts of the Flacourtia indica (Burm.f.) Merr. Fruits. Journal of Applied Research on Science and Technology 2020; 19(1):124-136.

Siramon P, Wongsheree T, Ultrasonic-assisted extraction of phenolic compounds from Paederia pilifera Hook. f. leaves and its antioxidant activity. Prawarun Agricultural Journal 2019; 16(1):182-189.

Yongpradoem P, Weerapreeyakul N. Evaluation of antioxidant activity and inhibition of tyrosinase activity of Raphanus sativus var. caudatus Alef Extract. Walailak Journal of Science and Technology 2020;17(8):838-850.




How to Cite

Khongpet, W., Sanghkawisu, P. ., Makerd, W. ., & Namjan, M. (2023). PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC CONTENTS AND ANTIOXIDANT ACTIVITY OF CURCUMA PUTII MAKNOI & JENJITT. EXTRACTS. Life Sciences and Environment Journal, 24(1), 14–25.



Research Articles