EFFICIENCY OF KERATINASE ENZYME PRODUCING BACTERIA ISOLATED FROM SOIL OF POULTRY FARMING FOR DEGRADATION OF CHICKEN FEATHER
Keywords:
feathers, enzyme, feather degrading bacteria, optimizing conditionsAbstract
In each year, Thailand has a large amount of feather left from the process of chicken meat production. Burning or land filling of those feathers can cause the air pollution. However, feather wastes can be potential used as substrates for digestible protein production by feather degrading microorganisms. This study, therefore, aimed to investigate the ability in feather degradation of 18 bacteria isolated (PSRU 1 – PSRU 18) from soil in Pathum poultry farm, Phitsanulok Province, which produced clear zone in skim milk agar plate. It was found that all bacteria were grown on raw feathers minimal medium and could degrade chicken feather in different levels. Further, gram strain, morphological studies and biochemical assays were used to identify the isolated feather degrading bacteria. The results showed 18 strains including, 9 isolates of Staphylococcus sp., 7 isolates of Bacillus sp., and 2 isolates of Corynebacterium sp.. The first three of high efficiency bacteria were selected to study the effects of temperature (30, 37, 40, 50 and 60°C) and pH values (4.5, 6.0, 7.5, 9.0, and 10.5) on degradation ability. The isolate PSRU 11 possessed 73.60% of degradation at 37°C and pH 7.5. Based on molecular identification, isolates PSRU 8, PSRU 11 and PSRU 12 were closely related to Bacillus cereus, B. subtilis and B. siamensis, respectively. The results from this study are likely to be applied for reducing feather wastes and could be a potential application in the production of protein source for animal feed.
References
Brosius J, Palmer L, Kennedy JP. et al. Complete nucleotide sequence of a 16s ribosomal RNA gene from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America. 1978; 75: 4801-4805.
Bockle B, Galunsky B, Muller R. Characterization of a kerationlytic serine protease from Streptomyces pactum DSM. 40530. Applied and Environmental Microbiology. 1995; 61: 3705-3710.
Eksittikul T, Kudan S. Isolation and partial purification of keratinase from novel bacteria isolated from poultry farm soil. Ramkhamhaeng Research Journal of Sciences and Technology. 2012; 15: 45-54.
Gupta R, Ramnani P. Microbial keratinases and their prospective applications: an overview. Applied and Environmental Microbiology. 2006; 70: 21-33.
Kim JM, Lim WJ, Suh HJ. Feather-degrading Bacillus species from poultry waste. Process Biochemistry. 2001; 37: 287-291.
Lane DJ, Pace B, Olsen G.J. et al. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis. Proceedings of the National Academy of Sciences U.S.A. 1985; 82: 6955–6959.
Laskhmi PJ, Chitturi ChMK, Lakshmi VV. Efficient degradation of feather by keratinase producing Bacillus sp. International Journal of Microbiology. 2013; 2013, 1-7.
Lin X, Kelemen DW, Miller ES. et al. Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. Applied and Environmental Microbiology. 1995; 61: 1469-1474.
Macedo AJ, Beys da Silva WO, Gava R. et al. Novel keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Applied and Environmental Microbiology. 2005; 71: 594-596.
Prasad HV, Kumar G, Karthik L. et al. Screening of extracellular keratinase producing bacteria from feather processing areas in Vellore, Tamil Nadu. Indian Journal of Scientific Research. 2010; 2: 559-565.
Riffel A, Brandelli A. Keratinolytic bacteria isolated from feather waste. Brazilian Journal of Microbiology. 2006; 37: 395-399.
Scott JA, Untereiner WA. Determination of keratin degradation by fungi using keratin azure. Medical Mycology. 2004; 42: 239-246.
Shih JCH, Williums CM. (1990). Feather-lysate, a hydrolyzed feather feed ingredient and animal feeds containing the same. US patent. 4, 908, 220. 4p.
Sivakumar T, Shankar T, Thangapandian V. et al. Optimization of cultural condition for keratinase production using Bacillus cereus TS1. Insight Microbiology. 2013; 1-8.
Sneath PHA, Mair NS, Sharpe ME. et al. Bergey’s manual of systematic bacteriology, vol 2 Baltimore: Williums and Wilkins. 1986. ISBN0-683-07893-3.
Wanchaitanawong P, Ritthitham S, Nitisinprasert S. Production of keratinase by Bacillus licheniformis KUB-K0006 and Bacillus pumilus KUB-K0082. Proceedings of the 38th Kasetsart University Annual Conference: Engineering, Agro-Industry. 2000; 522-528.
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