Influence of Sludge Sample Concentration on Heterotrophic Bacteria Yield Estimation by Using Oxygen Uptake Rate Measurement Method
Keywords:
Activated sludge, Yield, Heterotrophic, Respirometer, OURAbstract
The objective of this research is to study the effect of sludge concentration on heterotrophic bacteria yield (YH) by using oxygen uptake rate (OUR) with ultimate hybrid respirometer. The microorganisms for oxidation ditch process were collected from Maharaj Hospital, Nakhon Ratchasima Province, Thailand. We sampled the mixed liquor suspended solids (MLSS) from two difference sampling points; aeration tank at 4,370 mg/l, and return sludge pipe at 5,487 mg/l. Sludge samples were aerated overnight in the preparing chamber until reaching the endogenous respiration phase. MLSS sample concentrations were diluted to 880- 5,297 mg/L. Sodium acetate was used as a readily biodegradable COD substrate (rbCOD) and dosed to the MLSS sample. Final COD concentration was varied into 4 dilutions, at which they are between 7.21 -57.70 mg/L. The temperature, pH and dissolved oxygen (DO) were controlled throughout the experiment at 28±0.5 C, 7.8±0.1 and 6-7 mg/L, respectively.
Based on a simulation model of Activated sludge model 1 (ASM no.1) using liner regression and mass balance basic, the results showed that the MLSS concentrations from different sources have not performed any significant effects on heterotrophic bacteria yield (YH). i.e., the yield from (a) the returning sludge pipe and (b) the aerobic tank are 0.63 ± 0.00 mg/mg (r2 = 0.9934) and 0.56± 0.03 mg/mg (r2 = 0.9802), respectively. Time spending for OUR measurement for MLSS concentration of 880 and 5,297 mg/l to decompose COD concentration at 57.05 mg/l were 80 and 23 min. The difference of yield results due to the different time and sampling points, in which is a significant parameter, Spanjers et al. [1]. We also have found that the wastewater from hospitals were contaminated by drugs or other chemicals, possibly affect the microorganisms that are in the wastewater treatment plant.
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