Development of Primary Packaging Prototype for Control Anthracnose Disease of ‘Nam Dok Mai’ Mango Fruits during Transportation
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Abstract
Anthracnose disease of mangoes is regarded as a major postharvest disease in economic considerations, due to its potential impacts on shelf life limitations and consumer choices. Thus, the objective of this research was to investigate the application of electrospun poly(L-lactic acid) fiber mats containing silver nanoparticles (AgNPs) for controlling Colletotrichum gloeosporioides that causes anthracnose. Poly(L-lactic acid) (PLLA) fiber mats containing AgNPs concentrations 0, 1, 5, 10, 15% (w/w) were successfully prepared by electrospinning process. The minimum inhibitory concentration (MIC) values of AgNPs concentrations against C. gloeosporioides were determined by broth and agar dilution methods. As results, at the minimal concentration, AgNPs 10,000 ppm revealed the highest inhibition activity on C. gloeosporioides. AgNPs substances whereby antimicrobial properties were added to the neat PLLA solution in various concentrations, base on the weight of PLLA powder. Both the neat and the AgNPs-loaded PLLA solution were electrospun into ultra-fine fiber under a fixed electric field of 20 kV/18 cm. The obtained fibers were smooth, without the presence of any kind of aggregation on their surface. The average diameter of the neat PLLA fibers was 0.53 μm, while those of the AgNPs-loaded ones were in the range of 0.43 to 0.79 μm. Moreover, the antimicrobial activity of the 15% (w/w) AgNPs-loaded PLLA fiber mats was considered the greatest against C. gloeosporioides.
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