Reducing Sugar Production from Empty Fruit Bunches with Enzyme Cellic Ctec2®

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Published: May 18, 2018
Keywords:
Oil Palm Empty Fruit Bunch Glucose Xylose

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Anusith Thanapimmetha
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok
Suwatipat Tiyanusorn
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok
Penjit Srinophakun
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok
Maythee Saisriyoot
Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Bangkok

Abstract

Oil Palm Empty Fruit Bunch (OPEFB) was an agricultural residue that consists mostly of lignocellulosic material (57.88% cellulose, 23.81% hemicellulose and 16.51% lignin by weight). In this study, OPEFB was pretreated with 10, 15 and 20% (w/v) of sodium hydroxide for 60 minutes. The best pretreatment of OPEFB was 15% of sodium hydroxide. The result of pretreatment showed that the percentage of cellulose increased by 14.28% and the percentages of lignin decreased by 3.41%. After that, the pretreated OPEFB was hydrolyzed by Cellic Ctec2®enzyme with substrate loading of 5, 7, 10 and 15% (w/v), enzyme loading of 20 FPU/g substrate and 140 rpm for 5 days. The highest reducing sugar from OPEFB was 85.79 g·L–1 by using 10% substrate loading for 5 days. The reducing sugar consists of 60.29 g/L of glucose and 12.51 g/L of xylose by HPLC analysis.

Article Details

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Vol. 28 No. 2 (2018): April-June, 2018
Section
Engineering Research Articles

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