Gene Transmission Pattern of Salt Inducible Kinase1 (sik1(+/–)) Heterozygous Mutant in Zebrafish (Denio rerio)
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Abstract
Salt inducible kinase1 gene (sik1) plays an important role in controlling glucose and lipid homeostasis of metabolism pathway. This gene is an ovulation-related gene in zebrafish. The mutant zebrafish which their genome were modified by CRISPR/Cas9 may lead to irregular genetic transmission pattern of Mendel’s laws. This research aims to investigate gene transmission pattern in F2 zebrafish which was cross-breeding from F1 heterozygous mutants (sik1(+/–)) and wild type. Genotype of all survived F2 zebrafish were checked by heteroduplex mobility assay (HMA) and sequencing. The results showed that 12 bp insertion and 9 bp deletion at F2 heterozygous mutants (sik1(+/–)) could cause a stop codon after translation for 3 amino acids. The F1 heterozygous mutants (sik1(+/–)) could heritage their genotype to F2 heterozygous mutants (sik1(+/–)) but transmission pattern of sik1(+/–) was not in accordance with Mendel’s laws. Some of F2 heterozygous mutants (sik1(+/–)) may not survive due to the non-function of glucose and lipid homeostasis in metabolism pathway or abnormality during ovulation leading to non-Mendelian inheritance of gene transmission pattern. This research is a guide artic
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