The Development of Cultivation System of Microalgae with Outdoor Raceway Pond by Recirculating Water System
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Abstract
Batch and semi-continuous culture of freshwater microalgae Ankistrodesmus sp. BPR1102 were conducted outdoors in paddlewheel-driven raceway ponds implementing recirculating aquaculture systems. The used water from microalgae harvest tank was treated and recycled for microalgae cultivation. The medium were prepared from modified medium and cultured for 20 days. With batch cultivation, Ankistrodesmus sp. BPR1102 achieved the ma×imum cell density of 448×104 cells/ml and ma×imum specific growth rate of 0.67 day–1. During semi-continuous cultivation, addition of fresh medium and removal of microalgae cells were conducted. The dilution rate was varied from 0.50-0.77 day–1. The result showed that the highest cell density of 344×104 cells/ml was obtained at the lowest dilution rate (0.50 day–1). When the dilution rate was increased to 0.77 day–1, average cell density of the microalgae decreased to 109×104 cells/ml. However, the highest productivity was obtained at 0.57 day–1 dilution rate which provided cell productivity of 263×104 cells/ml/day (463 mg/L/day dry weight). The average lipid contents of 22% (dry weight) and average lipid productivity of 2,032 mg/L/day were obtained in semi-continuous cultivation.
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