ENHANCING GERMINATION AND SEEDLING GROWTH OF PEANUT TAINAN 9 VARIETY THROUGH SEED COATING WITH ENTEROBACTER KOBEI

Authors

  • Saralee Prommayakul Division of Agronomy, Faculty of Agricultural Production, Maejo University
  • Jiraporn Inthasan Bachelor of science (Soil Resources and Environment), Faculty of Agricultural Production, Maejo University
  • Vassana Viroonrat Bachelor of science (Soil Resources and Environment), Faculty of Agricultural Production, Maejo University
  • Jakkrapong Kangsopa Division of Agronomy, Faculty of Agricultural Production, Maejo University

Keywords:

Seed enhancement, Bio-fertilizer, Plant growth-promoting rhizobacteria, Bacteria-producing indole-3-acetic acid

Abstract

Peanuts can be grown year-round, yet the supply remains insufficient to meet consumer demand. This shortfall is primarily due to high cultivation costs from fertilizer use and poor-quality seeds, which result in uneven germination, weak seedlings, and susceptibility to pests and diseases. To address these issues, seed coating technology is essential for enhancing seed quality before planting. This study aimed to determine the optimal concentration of Enterobacter kobei for seed coating and to evaluate its effects on various seed quality parameters. The results indicated that coating seeds with E. kobei at 6.60x108 CFU/mL significantly improved germination rates and speed of germination compared to non-coated seeds under both sand and greenhouse conditions. Additionally, seeds coated with E. kobei at concentrations of 6.60x108 CFU/mL, 6.60x109 CFU/mL, and 6.60x1010 CFU/mL exhibited significantly greater shoot length, root length, seedling length, shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight in both testing conditions. In conclusion, coating peanut seeds with E. kobei at 6.60x108 CFU/mL is recommended for enhancing the germination, vigor, and growth of Tainan 9 peanut seedlings.

References

Agricultural Research Development Agency. (2017). Maize, Soybean, Mungbean and Peanut. The Direction of Thai Economic Crops in ASEAN. (2nd edition). Bankok: Pornsup Printing.

Anzuay, M.S., Prenollio, A., Ludueña, L.M., Morla, F.D., Cerliani, C., Lucero, C., Angelini, J.G., & Taurian, T. (2023). Enterobacter sp. J49: A native plant growth-promoting bacteria as alternative to

the application of chemical fertilizers on peanut and maize crops. Current Microbiology, 80(3), 85.

AOSA. (1983). Seed vigor testing handbook. USA: AOSA, Ithaca, NY.

Ehmann, A. (1977). The van Urk-Salkowski reagent - a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. Journal of Chromatography, 132(2), 267-276.

Ellis, R.A., & Roberts, E.H. (1980). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology. 9(2), 373-409.

Halmer, P. (2008). Seed technology and seed enhancement. Acta Horticulturae. 771, 17–26.

Inthasan, J., & Dechjiraratthanasiri, C. (2024). Influence of rhizobium co-inoculated with Arbuscular Mycorrhiza and phosphate solubilizing bacteria on the growth of peanut Tainan 9 variety. King Mongkut's Agricultural Journal, 42(1), 79-85.

ISTA. (2023). International Rules for Seed Testing. (Edition 2023). UK: International Seed Testing Association.

Jeephet, P., Atnaseo, C., Hermhuk, S., & Kangsopa, J. (2022). Effect of seed pelleting with different matrices on physical characteristics and seed quality of lettuce (Lactuca sativa). International Journal of Agricultural Technology, 18(5), 2009-2020.

Jomkhame, S., Kangsopa, J., & Atnaseo, J. (2022). Effects of phosphate and IAA of Burkholderia sp. and Enterobacter sp. on seed quality of Khao Dawk Mali 105 after soaking method. Burapha Science Journal, 27(1), 594-611.

Kangsopa, J. (2019). Seed coating. Maejo Journal of Agricultural Production, 1(2), 63-76.

Kumar, N., & Dubey, R.C. (2022). Plant growth promoting endophytic bacteria Bacillus australimaris BLR41 and Enterobacter kobei BLR45 enhance the growth of medicinal plant Barleria lupulina Lindl. Journal of Pure & Applied Microbiology, 16(4), 2647-2658.

Ludueña, L.M., Anzuay, M.S., Angelini, J.G., McIntosh, M., Becker, A., Rupp, O., Goesmann, A., Blom, J., Fabra, A., & Taurian, T. (2019). Genome sequence of the endophytic strain Enterobacter sp. J49, a potential biofertilizer for peanut and maize. Genomics, 111(4), 913-920.

Panneerselvam, P., Senapati, A., Sharma, L., Nayak, A.K., Kumar, A., Kumar, U., & Sagarika, M.S. (2021). Understanding rice growth-promoting potential of Enterobacter spp. isolated from long-term organic farming soil in India through a supervised learning approach. Current Research in Microbial Sciences, 2, 100035.

Pedrini, S., Merritt, D. J., Stevens, J., & Dixon, K. (2017). Seed coating: science or marketing spin?. Trends in Plant Science, 22, 106-116.

Pérez-García, L.A., Sáenz-Mata, J., Fortis-Hernández, M., Navarro-Muñoz, C.E., Palacio-Rodríguez, R., & Preciado-Rangel, P. (2023). Plant-growth-promoting rhizobacteria improve germination and bioactive compounds in cucumber seedlings. Agronomy, 13(2), 315.

Rocha, I., Ma, Y., Souza-Alonso, P., Vosátka, M., Freitas, H., & Oliveira, R.S. (2019). Seed coating: a tool for delivering beneficial microbes to agricultural crops. Frontiers in plant science, 10, 1357.

Roslan, M.A.M., Zulkifli, N.N., Sobri, Z.M., Zuan, A.T.K., Cheak, S.C., & Abdul Rahman, N.A. (2020). Seed biopriming with P-and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling. PloS one, 15(7), 0232860.

Sarron, E., Clément, N., Pawlicki-Jullian, N., Gaillard, I., & Boitel-Conti, M. (2018). Stimulating effects of two plant growth-promoting bacteria, Enterobacter ludwigii Ez-185-17 and Raoultella terrigena

Ez-555-6, on flax culture. AIP Conference Proceedings, 1954(1), AIP Publishing.

Scott, T.K. (1972). Auxins and roots. Annual Review of Plant Biology, 23, 235-258.

Siri, B. (2015). Seed conditioning and seed enhancements. Khon Kaen: Klungnanawitthaya Printing.

Vidak, M., Lazarević, B., Javornik, T., Šatović, Z., & Carović-Stanko, K. (2022). Seed water absorption, germination, emergence and seedling phenotypic characterization of the common bean landraces differing in seed size and color. Seeds, 1(4), 324-339.

Downloads

Published

2025-08-29

How to Cite

Prommayakul, S. ., Inthasan, J. . ., Viroonrat, V. . ., & Kangsopa, J. (2025). ENHANCING GERMINATION AND SEEDLING GROWTH OF PEANUT TAINAN 9 VARIETY THROUGH SEED COATING WITH ENTEROBACTER KOBEI. PSRU Journal of Science and Technology, 10(2), 41–53. retrieved from https://ph01.tci-thaijo.org/index.php/Scipsru/article/view/258041

Issue

Vol. 10 No. 2 (2025): May - August 2025

Section

Research Articles

License

Copyright (c) 2025 PSRU Journal of Science and Technology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

กองบรรณาธิการขอสงวนสิทธิ์ในการปรับปรุงแก้ไขตัวอักษรและคำสะกดต่างๆ ที่ไม่ถูกต้อง และต้นฉบับที่ได้รับการตีพิมพ์ในวารสาร PSRU Journal of Science and Technology ถือเป็นกรรมสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม และ
ผลการพิจารณาคัดเลือกบทความตีพิมพ์ในวารสารให้ถือมติของกองบรรณาธิการเป็นที่สิ้นสุด