Optimization of High Protein Content Protein Hydrolysate Extraction from Hard Clam (Meretrix casta) Using Response Surface Methodology

Abstract

The objective of this study was to investigate the optimum condition for high protein content protein hydrolysate extraction from hard clam using Response Surface Methodology. Three independent variables were ratio of water to hard clam meat, enzyme (protamex) concentration and extraction time. Box-Behnken design was used for experimental design. After protein hydrolysate production from hard clam, protein content was evaluated. The statistical analysis indicated two variables, linear of ratio of water to hard clam meat (X₁) and enzyme (protamex) concentration (X₂) had significant effects on protein content of protein hydrolysate (p<0.05). The optimum condition for high protein content protein hydrolysate (Y) were: ratio of water to hard clam meat was 1:8, enzyme (protamex) concentration was 1.5% and extraction time was 5.5 hours. Under these conditions, the predicted protein of protein hydrolysate was 84.97 mg albumin / ml protein hudrolysate.