Antifungal Activity of Chitinase from Seedlings of Plants
Keywords:
Antifungal activity, Chitinase, Plants seedlingsAbstract
Chitinase was enzyme that hydrolyze the β-1,4 glycosidics bond of chitin. Chitinase was found in many living organisms. However, chitinase extracted from plants had good ability to digest chitin, such as chitinase from Samanca saman (Jacq) Merr., Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630. Therefore, this research was to study the antifungal activity of chitinase from seedlings of Samanca saman (Jacq) Merr., Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630 of two weeks seedlings with extracted of 0.1 molar acetate buffer. It was found that specific enzyme activity of 10.8981, 9.7842, 8.3487 and 7.4132, U/mg were observed in Samanca saman (Jacq) Merr., Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630, respectively. The chitinase from Samanca saman (Jacq) Merr. had the highest specific activity, followed by Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630, respectively. The chitinase from Samanca saman (Jacq) Merr. had the most effective, completely inhibiting the growth of all six strains including Fusarium moniliforme, Geotrichum candidum, Mucor rouxii, Penicillium marneffei, Sporotrichum pulverulentum and Trichoderma reesei at a concentration of 0.25 mg/ml. The chitinase from Leucaena leucocephala de wit, Oryza sativa RD. 6 and Sorghum vulgare KU 630 effectively inhibited the growth of all six strains at a concentration of 0.5, 1.0 and 1.0 mg/ml, respectively.
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