Antibacterial Activity of Chitooligosaccharides Produced by Chitinase from Samanca saman (Jacq) Merr. Seedling
Keywords:
Antibacterial Activity, Chitooligosaccharides, Chitinase, Samanca saman (Jacq) Merr.Abstract
This research was to study the antibacterial activity of chitooligosaccharides obtained from hydrolysis of 1 percent of colloidal chitin produced by chitinase with extracted of 0.1 molar acetate buffer at pH 3.5 from two weeks seedlings of Samanca saman (Jacq). was investigate in this work. It found that chitooligosaccharides obtained by hydrolysis of 1 percent of colloidal chitin at 0.5 hour, containing a percentage of total large molecules of (GlcNAc)4, (GlcNAc)5, (GlcNAc)6, (GlcNAc)7, and (GlcNAc)8 was 92.58. It inhibited of the 6 bacterial strains, gram positive including Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and gram negative including Escherichia coli, Salmonella enterica, Vibrio parahaemolyticus. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 1.56/1.56, 3.12/3.12, 1.56/1.56, 1.56/3.12, 3.12/6.25 and 3.12/3.12 mg/ml, respectively.
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