ANTIBACTERIAL ACTIVITY AGAINST STAPHYLOCOCCUS AUREUS AND ANTIOXIDATION OF MEDICINAL PLANT EXTRACT
DOI:
https://doi.org/10.14456/lsej.2024.7Keywords:
medicinal plant, skin disease, antibacterial, antioxidantAbstract
The study of antibacterial activity against Staphylococcus aureus and antioxidation of medicinal plant extract was done by three medicinal plants which selected from Thai traditional medicine textbooks, namely Rhinacanthus nasutus (L.) Kurz, Piper betle L. and Cassia fistula L. They were extracted using water and 80% ethanol. The extractions determined antibacterial activity against S. aureus by agar disc diffusion at 10 and 100 mg/mL. The results showed the highest activity of 100 mg/mL 80% ethanol extract of Piper betle L. leaves to inhibit bacterial growth with an inhibition diameter of 2.45±0.08 cm. The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The MBC to MIC ratio of Piper betle L. leaves water extract was 0.50, which showed the highest bactericidal activity compared with other medicinal plant extract. While the 80% ethanol extract of Piper betle L. leaves, Cassia fistula L. leaves and water extract of Cassia fistula L. leaves showed the MBC to MIC ratio of 8.01, 16.03 and 32.05, respectively. Those represented the bacteriostatic activity. Moreover, the antioxidants of all extracts were detected by a DPPH assay. Water extract of Piper betle L. leaves showed the highest antioxidant activity with IC50 3.12±0.06 mg/mL. This research data supported the use of medicinal plants in skin disease treatment by Thai traditional medicine. It also showed the opportunity to develop medicinal plant products.
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