PLANT GROWTH PROMOTING ACTIVITIES OF ACTINOMYCETES ISOLATED FROM MEALWORM FRASS AND SUPERWORM FRASS
DOI:
https://doi.org/10.14456/lsej.2024.4Keywords:
plant growth promoting bacteria, actinomycetes, mealworm frass, superworm frassAbstract
Actinomycetes are recognized as the plant growth promoting bacteria (PGPB).
A total of 24 actinomycetes were isolated from mealworm frass and superworm frass. The evaluation of plant growth promoting activities of these actinomycetes found that 22 isolates were able to produce the enzyme ACC Deaminase, 18 isolates were able to produce siderophore, eight isolates were able to solubilize phosphate and 20 isolates were capable of producing high amount of IAA plant hormone (23.59 – 309.15 µg/ml), effectively promoting the root growth of Pathum Thani 1 rice seeds by increasing root length and volume compared to control and non IAA-producing isolates. However, among these, only six isolates (TM1, TM4, TM6, TM8, ZA1, and ZA3) exhibited positive results in all tested biochemical activities. Based on nucleotide sequence analysis and the phylogenetic relationship of the partial 16S rRNA gene, these six isolates were identified as belonging to the genus Streptomyces. These findings could be promising for utilizing worm frass directly as organic fertilizer or isolating actinomycetes as bio-fertilizer substances to enhance plant growth.
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