• Chakkrit Sreela-or Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University
  • Punnada Tharungsri Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University
  • Supawadee Yamkong Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University
  • Patthanun Kotam Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University
  • Prapasiri Jaipong Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University




Optimization , Bio-extract, Marigold


Factors affecting yields of marigold using fermented bio-extract from urine with vinasse and animal manure were analyzed by response surface methodology with a central composite design. The various concentrations of each bio-extract concentration and volume of bio-extract were investigated. The results revealed that the optimum conditions were bio-extract of 16.38 %v/v and volume of bio-extract of 40.13 mL/plant. Under the optimal conditions, the maximum flower number, flower size and flower fresh weight were found at 7.84 flowers/plant,
5.90 cm and 7.34 g/flower, respectively. The confirmation experiment under the optimal condition showed the flower number of 8.00 flowers/plant, flower size of 5.98 cm and flower fresh weight of 7.38 g/flower. The result was close to the predicted values by linear regression.


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How to Cite

Sreela-or, C., Tharungsri, P. ., Yamkong, S. ., Kotam , P. ., & Jaipong, P. . (2022). OPTIMUM CONDITIONS OF USING BIO-EXTRACT FROM URINE WITH VINASSE AND MANURE AFFECTING YIELDS OF MARIGOLD. Life Sciences and Environment Journal, 23(2), 283–296. https://doi.org/10.14456/lsej.2022.22



Research Articles